| Accession |
PRJCA030099 |
| Title |
A novel protein NSD1-916aa encoded by CircNSD1 contributes to AKI-to-CKD transition through inducing ferroptosis in renal tubular epithelial cells-4 |
| Relevance |
Medical |
| Data types |
metabonomics
|
| Organisms |
Homo sapiens
|
| Description |
Lipid extraction from H/R-induced control and CircNSD1-overexpressing cells was performed using a standardized protocol. Quality control (QC) samples were prepared by pooling equal volumes of extracts from all samples, ensuring that the volume of each QC sample was equivalent to that of the individual samples. Subsequently, the samples were subjected to separation using a Nexera UPLC system (Shimadzu) and analyzed via a Q Exactive mass spectrometer (Thermo Scientific). The LC/MS detection was carried out at Lumingbio Company. To identify differential lipid metabolites, a supervised multiple regression orthogonal partial least squares discriminant analysis (OPLS-DA) model was applied. To mitigate the risk of over-fitting, permutation testing was conducted with 200 iterations. The resulting data were deposited into a relevant database for further analysis and reference. |
| Sample scope |
Monoisolate |
| Release date |
2024-09-12 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| the National Natural Science Foundation
|
|
82300820
|
|
| the China Postdoctoral Science Foundation
|
|
2023T160001
|
|
| the China Postdoctoral Science Foundation
|
|
2022M710179
|
|
|
|
| Submitter |
Li
Gao (18056052029@163.com)
|
| Organization |
The First Affiliated Hospital of Anhui Medical University |
| Submission date |
2024-09-12 |
