| 项目编号 |
PRJCA030207 |
| 项目标题 |
Highly Efficient Construction of Monkey Blastoid Capsules from Aged Somatic Cells |
| 涉及领域 |
Evolution |
| 数据类型 |
Single cell sequencing
|
| 物种名称 |
Macaca fascicularis
|
| 描述信息 |
This study reports the highly efficient generation of blastoids from induced pluripotent stem cells (iPSCs) and somatic cell nuclear transfer ESCs (nt-ESCs) obtained from aged monkeys. With our protocol we achieve a blastoid formation efficiency of up to 80%. Additionally, we developed a hydrogel-based microfluidics platform to produce size-adjustable, biodegradable and monodisperse blastoid capsules in a scalable and reproducible manner. The capsules offer a stable 3D structure and mechanical protection during delivery through fallopian tubes. The high efficiency and scalable production of monkey blastoid capsules from reprogrammed aged somatic cells provides a powerful tool to advance the studies of embryonic development and may have broader implications for regenerative medicine. |
| 样品范围 |
Single cell |
| 发布日期 |
2024-09-14 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 39875393
|
Highly efficient construction of monkey blastoid capsules from aged somatic cells
|
Nature Communications
|
10.1038/s41467-025-56447-z
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
U2102204
|
|
| National Natural Science Foundation of China (NSFC)
|
|
52202350
|
|
|
|
| 提交者 |
Zhao
Bo (kiz_zhaolab@163.com)
|
| 提交单位 |
Kunming Institute of Zoology, Chinese Academy of Sciences |
| 提交日期 |
2024-09-14 |
