| 项目编号 | PRJCA030517 | ||||||||
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| 项目标题 | Unveiling Early Microbial Colonization: 2bRAD-M Enables the Analysis of Low-Biomass Microbiome in Maternal and Infant Microbiome | ||||||||
| 涉及领域 | Environmental | ||||||||
| 数据类型 |
Whole genome sequencing
Metagenome Raw sequence reads Genome sequencing |
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| 物种名称 |
human milk metagenome
human gut metagenome |
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| 描述信息 | The microbial composition of human breast milk and infant meconium offers critical insights into early microbial colonization, contributing to the infant's immune system and long-term health outcomes1. However, analyzing these samples often faces technical challenges and problems of low-resolution using conventional methods due to their low microbial biomass. Here, we employed type IIB restriction enzymes site-associated DNA sequencing for microbiome (2bRAD-M), a reduced metagenomics method, to address these issues and profile species-level microbial composition. We collected samples of breast milk, maternal feces, and infant meconium and compared 2bRAD-M against the commonly used 16S rRNA amplicon sequencing and the gold-standard whole metagenomics sequencing (WMS). The accuracy and robustness of 2bRAD-M were demonstrated through its consistently high correlation in bacterial individual abundance and low whole-community-level distance with paired WMS samples. Moreover, with 2bRAD-M, we observed clinical variables associated with infant microbiota variations and significant microbial diversity changes across different lactation stages in breast milk. This study underscores the importance of employing 2bRAD-M in future large-scale and longitudinal studies of maternal and infant microbiomes, enhancing our understanding of microbial colonization and transmission in early life stages. | ||||||||
| 样品范围 | Environment | ||||||||
| 发布日期 | 2025-01-09 | ||||||||
| 项目资金来源 |
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| 提交者 | Yuesong Jiang (jiang666@connect.hku.hk) | ||||||||
| 提交单位 | The Uinversity of Hong Kong | ||||||||
| 提交日期 | 2024-09-25 |
| 资源名称 | 描述 |
|---|---|
| BioSample (247) show | - |
| GSA (5) | - |
| CRA022259 | WMS sequencing data of human fecal samples |
| CRA021477 | 16S sequencing data of human milk samples |
| CRA021480 | 16S sequencing data of human gut samples |
| CRA019237 | 2bRAD-M sequencing data of human milk samples |
| CRA019236 | 2bRAD sequencing data of human gut samples |