| Accession |
PRJCA030775 |
| Title |
Understory growth of important Himalayan medicinal plant, Paris polyphylla accumulates a reservoir of plants secondary metabolites |
| Relevance |
Medical |
| Data types |
metabolism
|
| Organisms |
Paris polyphylla
|
| Description |
Paris polyphylla is an important traditional medicinal plant in the Himalayan region. It is extensively used for the production of natural steroidal saponins and flavonoids. Although, seed dormancies of wild plant can be broken to artificially maintain and regenerate through micropropagation in the laboratories. However, success of secondary metabolites production in higher quantity and synthesis of superior plant metabolites have been very limited. In this study, we present differential metabolic profiling of Paris polyphylla plants maintained for eight years in natural as well as greenhouse conditions. Untargeted profiling of metabolites through ultra-performance liquid chromatography/mass spectrometry, followed by statistical analysis, identified secondary metabolites that were enriched in naturally occurring plant roots, compared with greenhouse plant roots. Multivariate statistical analysis revealed the differential distribution of compounds between the two groups. Overall, we identified 1182 secondary metabolites, where 116 metabolites were differentially up regulated whereas 256 metabolites were down regulated. Whereas, 810 metabolites insignificantly varied in both the growing conditions. Koyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that naturally forest grown Paris plants were significantly enriched in steroidal saponin, lipids, vitamins, flavonoids and flavonols. Analysis of top-10 differentially up-regulated secondary metabolites indicated significantly enriched quantity of spirost-5-en-3,12-diol and Kaempferol synthesis pathways, which are considered as free radical scavengers inside the cell. Additionally veratramine alkaloids were also enriched in natural conditions. We show that the naturally maintained Paris plants are suitable for extraction of medicinally important compounds. Our study establishes the causal relationship between the metabolic composition of the roots and their natural growth condition. This study highlights the importance of environmental conditions in biosynthesis of plant secondary metabolites. |
| Sample scope |
Different growing environments |
| Release date |
2024-10-01 |
| Publication |
|
| Data provider |
|
| Biomaterial provider |
Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, International Ecological Foresty Research Center of Kunming, Southwest Forestry University |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
32260720
|
This work was supported by National Key R&D Program of China(2021YFD1000202), China Agriculture Research System of MOF & MARA (CARS-21-05B), National Natural Science Foundation of China(32260720)
|
|
|
| Submitter |
rui
Shi (11566221@qq.com)
|
| Organization |
Southwest Forestry University |
| Submission date |
2024-10-01 |
