| 项目编号 |
PRJCA030984 |
| 项目标题 |
Single-cell dissection of intratumoral heterogeneity and developmental lineages in early-stage lung adenocarcinoma |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
Single cell sequencing
|
| 物种名称 |
|
| 描述信息 |
Intratumoral heterogeneity (ITH) has been investigated primarily in locally advanced or metastatic cancer; however, much less is known about ITH in early-stage cancer, and the origins of ITH are poorly understood. We performed single-cell transcriptome profiling of early-stage lung adenocarcinoma from 12 patients, constructed a map of 88,695 cells, profiled the transcriptome states of tumor cell populations, and incisively explored the ITH of neoplastic cells. Significant transcriptional ITH is already present in early-stage lung adenocarcinoma (12 of 12 patients). The observed heterogeneity originates from two main sources. On the one hand, during tumor evolution, epithelial dedifferentiation can serve as a predictor of prognosis. On the other hand, early-stage tumor cells retain the original AT2 lineage differentiation program, representing an intermediate transition state from AT2 to AT1 cells. Two key transcription factors KLF4 and JDP2 were identified, overexpression of which in late-stage LUAD reverts tumor cells to this intermediate transitional state. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2024-10-14 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
General Program
|
82072974
|
|
| National Natural Science Foundation of China (NSFC)
|
General Program
|
82120108020
|
|
| National Natural Science Foundation of China (NSFC)
|
General Program
|
81572642
|
|
| National Key Research and Development Program of China
|
General Program
|
2023YFE0117900
|
|
| Innovation Program of Shanghai Science and technology committee
|
General Program
|
20Z11900300
|
|
| Postdoctoral Fellowship Program of CPSF
|
General Program
|
GZB20240790
|
|
|
|
| 提交者 |
Yuexiang
Wang (yxwang76@sinh.ac.cn)
|
| 提交单位 |
Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences |
| 提交日期 |
2024-10-09 |
