| 项目编号 |
PRJCA031160 |
| 项目标题 |
Reconstitution of spermatogenesis and continuous generation of functional haploid germ cells in mouse testicular organoids |
| 涉及领域 |
Model organism |
| 数据类型 |
Single cell sequencing
|
| 物种名称 |
|
| 描述信息 |
Testicular organoids that support spermatogenesis and generate functional haploid germ cells are still lacking. Here, we developed a formation-differentiation culture approach to generate optimized testicular organoids (O-Torgs) derived from neonatal mouse primary testicular cells. These O-Torgs could essentially recapitulate the seminiferous tubule morphogenesis, Sertoli cell maturation and testosterone secretion. Importantly, O-Torgs not only support spermatogenesis but also sustain proliferative undifferentiated spermatogonia and continuous generation of functional haploid cells for up to three months, with offspring derived from these haploid cells displaying normal growth and reproductive capability to F2 generation. Mechanistically, we found that the formation stage enhanced the reconstruction of tubule-like structures and subsequent spermatogenesis by providing a more conducive extracellular matrix niche. Finally, O-Torgs were proved to be effective in modeling male infertility and drug screening, with BTT-3033 acting as a potential drug in protecting busulfan-mediated germ cell loss. Overall, our work establishes a strategy to obtain functional testicular organoids, offering promising avenues for male infertility modeling and drug discovery. |
| 样品范围 |
Single cell |
| 发布日期 |
2026-03-08 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 41912581
|
Reconstitution of spermatogenesis and continuous generation of functional haploid germ cells in mouse testicular organoids
|
Nature Communications
|
10.1038/s41467-026-71254-w
|
2026
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
82071711
|
|
| National Natural Science Foundation of China (NSFC)
|
|
31970787
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32170869
|
|
| Shenzhen Science and Technology Innovation Commission
|
|
JCYJ20210324120212033
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32200670
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32200689
|
|
| China Postdoctoral Science Foundation
|
|
2022M711526
|
|
|
|
| 提交者 |
Xiaoyang
Zhao (zhaoxiaoyang@smu.edu.cn)
|
| 提交单位 |
Southern Medical University |
| 提交日期 |
2024-08-19 |
