| 项目编号 |
PRJCA031427 |
| 项目标题 |
Histone lactylation drives liver cancer metastasis by facilitating NSF1 mediated ferroptosis resistance after microwave ablation |
| 涉及领域 |
Medical |
| 数据类型 |
Proteome
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
The separation was performed on an 1100 HPLC System (Agilent) using a Nano Chrom-C18 column. Mobile phases A (2% acetonitrile in HPLC water) and B (98% acetonitrile in HPLC water) were used for RP gradient. Tryptic peptides were separated at an fluent flow rate of 300 μL/min and monitored at 210 nm. Samples were collected for 8-53 minutes, and eluent was collected in centrifugal tube 1-15 every minute in turn.Samples were recycled in this order until the end of gradient. The separated peptides were lyophilized for mass spectrometry. All analyses were performed by a Fusion mass spectrometer equipped with a Easyspray source. Samples were loaded and separated by a capillary analytical column on an EASY-nLCTM 1200 system.The flow rate was 300 nL/min. Full MS scans were acquired in the mass range of 350- 1800 m/z with a mass resolution of 60000 and the AGC target value was set at 4e5. The 10 most intense peaks in MS were fragmented with higher-energy collisional dissociation with collision energy of 35. MS/MS spectra were obtained with a resolution of 15000 with an AGC target of 5e4 and a max injection time of 22 ms. The Fusion dynamic exclusion was set for 60.0 s and run under positive mode. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2025-12-01 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 39970777
|
Histone lactylation drives liver cancer metastasis by facilitating NSF1-mediated ferroptosis resistance after microwave ablation
|
Redox Biology
|
10.1016/j.redox.2025.103553
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China
|
|
82171952
|
|
|
|
| 提交者 |
Jiayan
yan
Huang (Sue66joo@163.com)
|
| 提交单位 |
West China Hospital of Sichuan University |
| 提交日期 |
2024-10-21 |
