项目编号

PRJCA031498

项目标题

Genome-wide profiling the integration pattern with T7-PCR

涉及领域

Model organism

数据类型

Whole genome sequencing
Map
Targeted Locus (Loci)
Raw sequence reads

物种名称

Oryza sativa
Arabidopsis
Glycine max
Saccharomyces cerevisiae

描述信息

In this study, we demonstrated a new method, T7-PCR, for insertion sites mapping from genome. T7-PCR exhibits excellent accuracy and efficiency in resolving complex integration events in yeast, plant, and mammalian cells. We established the whole experimental procedure and optimized experimental parameters, demonstrating its university and reliability in common integration scenarios.

样品范围

Multispecies

发布日期

2025-07-24

项目资金来源

机构	项目类型	授权项目ID	授权项目名称
Natural Science Foundation of Guangdong Province		2024A1515012107

提交者

Fannv Xia (xiafn@mail.sysu.edu.cn)

提交单位

Sun Yat-sen University

提交日期

2024-10-23

项目包含数据信息

资源名称	描述
BioSample (58) show	-
SAMC4680386	Mapping T-DNA insertion sites of soybean transgenic root G12
SAMC4680385	Mapping T-DNA insertion sites of soybean transgenic root G10
SAMC4680384	Mapping T-DNA insertion sites of soybean transgenic root G9
SAMC4680383	Mapping T-DNA insertion sites of soybean transgenic root G7
SAMC4680382	Mapping T-DNA insertion sites of soybean transgenic root G6
SAMC4680381	Mapping T-DNA insertion sites of soybean transgenic root G3
SAMC4680380	Mapping T-DNA insertion sites of soybean transgenic root G2
SAMC4680379	Mapping T-DNA insertion sites of soybean transgenic root G1
SAMC4680378	Mapping T-DNA insertion sites of rice transgenic line R40
SAMC4680377	Mapping T-DNA insertion sites of rice transgenic line R39
SAMC4680376	Mapping T-DNA insertion sites of rice transgenic line R31
SAMC4680375	Mapping T-DNA insertion sites of rice transgenic line R24
SAMC4680374	Mapping T-DNA insertion sites of rice transgenic line R15
SAMC4680373	Mapping T-DNA insertion sites of rice transgenic line R10
SAMC4680372	Mapping T-DNA insertion sites of rice transgenic line O22
SAMC4680371	Mapping T-DNA insertion sites of rice transgenic line O20
SAMC4680370	Mapping T-DNA insertion sites of rice transgenic line O19
SAMC4680369	Mapping T-DNA insertion sites of rice transgenic line O17
SAMC4680368	Mapping T-DNA insertion sites of rice transgenic line O14
SAMC4680367	Mapping T-DNA insertion sites of rice transgenic line O12
SAMC4680366	Mapping T-DNA insertion sites of rice transgenic line O11
SAMC4680365	Mapping T-DNA insertion sites of rice transgenic line O10
SAMC4680364	Mapping T-DNA insertion sites of rice transgenic line O9
SAMC4680363	Mapping T-DNA insertion sites of rice transgenic line O8
SAMC4680362	Mapping T-DNA insertion sites of rice transgenic line O2
SAMC4680361	Mapping T-DNA insertion sites of rice transgenic line O1
SAMC4680360	Mapping T-DNA insertion sites of Arabidopsis transgenic line B30
SAMC4680359	Mapping T-DNA insertion sites of Arabidopsis transgenic line B25
SAMC4680358	Mapping T-DNA insertion sites of Arabidopsis transgenic line B18
SAMC4680357	Mapping T-DNA insertion sites of Arabidopsis transgenic line B14
SAMC4680356	Mapping T-DNA insertion sites of Arabidopsis transgenic line B13
SAMC4680355	Mapping T-DNA insertion sites of Arabidopsis transgenic line B10
SAMC4680354	Mapping T-DNA insertion sites of Arabidopsis transgenic line B2
SAMC4680353	Mapping T-DNA insertion sites of Arabidopsis transgenic line A25
SAMC4680352	Mapping T-DNA insertion sites of Arabidopsis transgenic line A18
SAMC4680351	Mapping T-DNA insertion sites of Arabidopsis transgenic line A13
SAMC4680350	Mapping T-DNA insertion sites of Arabidopsis transgenic line A11
SAMC4680349	Mapping T-DNA insertion sites of Arabidopsis transgenic line A9
SAMC4680348	Mapping T-DNA insertion sites of Arabidopsis transgenic line A8
SAMC4680347	Mapping T-DNA insertion sites of Arabidopsis transgenic line A7
SAMC4680346	Mapping T-DNA insertion sites of Arabidopsis transgenic line A5
SAMC4680345	Mapping T-DNA insertion sites of Arabidopsis transgenic line A4
SAMC4680344	Mapping T-DNA insertion sites of Arabidopsis transgenic line A3
SAMC4680343	Mapping T-DNA insertion sites of Arabidopsis transgenic line A1
SAMC4284664	yeast packaged with PiggyBac system; inducing-expression of transposase for 6 hours; colony S5
SAMC4284663	yeast packaged with PiggyBac system; inducing-expression of transposase for 6 hours; colony S4
SAMC4284662	yeast packaged with PiggyBac system; inducing-expression of transposase for 6 hours; colony S3
SAMC4284661	yeast packaged with PiggyBac system; inducing-expression of transposase for 6 hours; colony B4
SAMC4284660	yeast packaged with PiggyBac system; inducing-expression of transposase for 48 hours; colony S5
SAMC4284659	yeast packaged with PiggyBac system; inducing-expression of transposase for 48 hours; colony S3
SAMC4284658	yeast packaged with PiggyBac system; inducing-expression of transposase for 48 hours; colony S2
SAMC4284657	yeast packaged with PiggyBac system; inducing-expression of transposase for 48 hours; colony S1
SAMC4284656	yeast packaged with PiggyBac system; inducing-expression of transposase for 48 hours; colony B5
SAMC4284655	yeast packaged with PiggyBac system; inducing-expression of transposase for 48 hours; colony B4
SAMC4284654	yeast packaged with PiggyBac system; inducing-expression of transposase for 48 hours; colony B1
SAMC4284653	yeast with two targeted-integrations mediated by HDR, colony 3
SAMC4284652	yeast with two targeted-integrations mediated by HDR, colony 2
SAMC4284651	yeast with two targeted-integrations mediated by HDR, colony 1
GSA (2)	-
CRA022775	T7-PCR in Plants
CRA019918	T7-PCR in Yeast