| Accession | PRJCA031954 | ||||||||||||||||||||
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| Title | The functional analysis of rice 3-ketoacyl-CoA synthase 11 gene reveals a metabolic pathway shift from very long chain fatty acids to cytokinin | ||||||||||||||||||||
| Relevance | Agricultural | ||||||||||||||||||||
| Data types |
Whole genome sequencing
Phenotype or Genotype Raw sequence reads Genome sequencing |
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| Organisms | Oryza sativa | ||||||||||||||||||||
| Description | The rice cultivar Nipponbare (Oryza sativa L. japonica) and Chengdian semi-dwarf-1 (csd-1) were used in this study. Following previous studies, we based our approach on the protein-coding genes identified in the MSU (Michigan State University) rice genome database (https://rice.uga.edu/). For each target gene, we designed two specific editing sites to ensure coverage and efficiency in gene disruption. The Cas9 editing library was then constructed, targeting these sites within each gene, allowing for precise and efficient editing. We applied a library of CRISPR-Cas9 constructs to generate an edited rice pool in prophase. After more than three generations of self-pollination, we achieved a non-transgenic population exhibiting normal phenotypes. We selected two semi-dwarf mutants and six normal-height individuals from the T2 segregating generation lines. Whole-genome sequencing were then performed on these lines. | ||||||||||||||||||||
| Sample scope | Multiisolate | ||||||||||||||||||||
| Release date | 2024-11-04 | ||||||||||||||||||||
| Grants |
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| Submitter | Yangshuo Han (han_ysys@163.com) | ||||||||||||||||||||
| Organization | Southwest University | ||||||||||||||||||||
| Submission date | 2024-11-01 |