| 项目编号 | PRJCA032153 | ||||||||||||||||
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| 项目标题 | A multifaceted role of INTS12-dependent Pol II removal in transcription-coupled repair | ||||||||||||||||
| 涉及领域 | Medical | ||||||||||||||||
| 数据类型 |
Epigenomics
Transcriptome or Gene expression Raw sequence reads |
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| 物种名称 | |||||||||||||||||
| 描述信息 | During the process of transcription-coupled nucleotide excision repair (TC-NER), damage-stalled RNA polymerase II (Pol II) must be displaced from DNA lesions to allow repair by downstream NER factors.However, the mechanisms underlying the release of stalled Pol II remain incompletely understood. In this study, we identify the Integrator complex as a critical mediator of Pol II removal to ensure efficient TC-NER. INTS12, a flexible subunit of the Integrator complex with previously uncharacterized functions, is rapidly phosphorylated in response to UV irradiation.This phosphorylation enhances INTS12 interaction with CSB, promoting the recruitment of the Integrator complex to facilitate the release of lesion-stalled Pol II and subsequent DNA repair. Disruption of this INTS12-mediated pathway impairs transcription recovery and TC-NER, leading to reduced cellular resistance to UV-induced stress. Interestingly, the INTS12-coordinated removal of stalled Pol II is less favorable for transcription-coupled DNA-protein crosslink (TC-DPC) repair, which relies on proteolytic degradation of stalled Pol II for DPC removal. | ||||||||||||||||
| 样品范围 | Monoisolate | ||||||||||||||||
| 发布日期 | 2026-01-24 | ||||||||||||||||
| 项目资金来源 |
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| 提交者 | song hua lu (huasong_lu@zju.edu.cn) | ||||||||||||||||
| 提交单位 | Zhejiang University | ||||||||||||||||
| 提交日期 | 2024-11-08 |
| 资源名称 | 描述 |
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| BioSample (70) show | - |