| 项目编号 | PRJCA032355 | ||||||||||||||||||||||||||||||||||||||||
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| 项目标题 | Molecular and structural basis of plant DCL4 action that outcompetes DCL2 | ||||||||||||||||||||||||||||||||||||||||
| 涉及领域 | Agricultural | ||||||||||||||||||||||||||||||||||||||||
| 数据类型 |
Transcriptome or Gene expression
small RNA sequencing |
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| 物种名称 | |||||||||||||||||||||||||||||||||||||||||
| 描述信息 | Small interfering RNAs (siRNAs) regulate eukaryotic development and immunity. Plant DICER-LIKE 2 (DCL2) and DCL4 produce 22-nucleotide and 21-nucleotide siRNAs, respectively. DCL4 outcompetes DCL2 to prevent the uncontrolled generation of 22-nucleotide siRNAs, which can produce secondary siRNAs and widespread, detrimental gene silencing. Here, we identified the structural features of Arabidopsis DCL4 that confer minimal specificity for double-stranded RNA (dsRNA) termini and a preference for cleaving long dsRNAs, allowing it to process diverse dsRNAs. The second dsRNA binding domain (dsRBD2) of DCL4 interacts with DRB4. Fusing dsRBD2 to DCL2 enhanced its activity, leading to growth defects and activated stress responses. Our findings uncover the mechanism of DCL4 action and highlight the role of the dsRBD2-DRB4 module in enabling DCL4 to outcompete DCL2. | ||||||||||||||||||||||||||||||||||||||||
| 样品范围 | Multiisolate | ||||||||||||||||||||||||||||||||||||||||
| 发布日期 | 2026-01-30 | ||||||||||||||||||||||||||||||||||||||||
| 项目资金来源 |
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| 提交者 | Wei Yan (yanwei_bio@126.com) | ||||||||||||||||||||||||||||||||||||||||
| 提交单位 | Southern University of Science and Technology | ||||||||||||||||||||||||||||||||||||||||
| 提交日期 | 2024-11-14 |