| 项目编号 |
PRJCA032510 |
| 项目标题 |
A gut microbiota-bile acid axis inhibits infection of an emerging coronavirus by targeting its cellular receptor aminopeptidase N |
| 涉及领域 |
Agricultural |
| 数据类型 |
Metabolome
|
| 物种名称 |
Sus scrofa
|
| 描述信息 |
Our results revealed that PDCoV infection causes microbiota dysbiosis in pigs, significantly reducing the intestinal abundance of Bacteroides fragilis, which correlated with disruptions in bile acid metabolism. Colonization with bile salt hydrolase (BSH)-producing B. fragilis increased the load of unconjugated bile acids and inhibited PDCoV infection, highlighting the role of microbiota-associated bile acid metabolism in viral pathogenesis. LCA, a prominent unconjugated bile acid, was shown to effectively inhibit PDCoV infection in porcine small intestinal epithelial cells and porcine intestinal enteroids. Notably, LCA inhibited PDCoV replication independently of bile acid receptor signaling and innate immune modulation. Mechanistic studies indicated that LCA prevents PDCoV infection by disrupting the viral entry process, specifically inhibiting binding between the PDCoV spike protein and its cellular receptor, aminopeptidase N. In vivo experiments further confirmed that LCA significantly inhibits PDCoV infection in piglets. |
| 样品范围 |
Environment |
| 发布日期 |
2024-11-22 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
|
|
A gut microbiota‐bile acid axis inhibits the infection of an emerging coronavirus by targeting its cellular receptor aminopeptidase N
|
iMeta
|
10.1002/imt2.70061
|
2025
|
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| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| No funding support
|
|
|
|
|
|
| 提交者 |
Jinxin
Meng (mengjx855@163.com)
|
| 提交单位 |
College of Animal Sciences, Zhejiang University |
| 提交日期 |
2024-11-18 |
