| 项目编号 |
PRJCA033138 |
| 项目标题 |
Based on metabolomics analysis: metabolic mechanism of intestinal tract of Scylla paramamosain under low-salt saline-alkali water aquaculture environment |
| 涉及领域 |
Agricultural |
| 数据类型 |
metabonomics
|
| 物种名称 |
Scylla paramamosain
|
| 描述信息 |
Metabolomic analysis was performed using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry (LCMS) following established protocols.The chromatography was performed on ACQUITY UPLC HSS T3 column in positive and negative modes.The gradient elution system consists of (A) water (containing 0.1% formic acid, v/v) and (B) acetonitrile (containing 0.1% formic acid, v/v) using the following gradients.Programmed temperature rise: the initial temperature of the column temperature box is 60 and maintained for 0.5min;Heat up to 125 at 8 /min; 8 /min to 210 ; 15 /min to 270 ; Temperature rise from 20 /min to 305 for 5 min. Electron bombardment ion source (EI), ion source temperature 230 , quadrupole temperature 150 , electron energy 70 eV. Scanning mode is full scanning mode (SCAN), quality scanning range: m/z 50-500.Data analysis:Using orthogonal partial least squares analysis to filter out noise unrelated to classification information, improving the analytical ability and effectiveness of the model, and maximizing the differences between different groups within the model. To prevent overfitting of the model, seven-fold cross validation and 200 response permutation testing were used to evaluate the quality of the model. Differential metabolites with VIP value greater than 1.0 and p value less than 0.05 were selected. Using KEGG compound data Annotate identified metabolites and then map the annotated metabolites to the KEGG pathway database. For a given list of metabolites, use the p-value of hypergeometric test to identify significantly enriched pathways. |
| 样品范围 |
intestinal structure of Scylla Paramamosain |
| 发布日期 |
2024-12-03 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
|
|
Based on metabolomics analysis: metabolic mechanism of intestinal tract of Scylla paramamosain under low-salt saline-alkali water aquaculture environment
|
BMC Genomics
|
10.1186/s12864-024-11138-9
|
2024
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China
|
|
42276106
|
|
| the Zhejiang Agricultural Science and Technology Cooperation Project
|
|
2024SNJF073
|
|
| the Science and Technology Program of Yulin city, ShanXi province
|
|
2023-CXY-220
|
|
| the Public Welfare Research Project of Ningbo
|
|
2023S114
|
|
| the earmarked fund for CARS
|
|
CARS-48
|
|
| the youth science and technology innovation leading talent project of Ningbo city
|
|
2023QL038
|
|
|
|
| 提交者 |
Chenxi
Che (1798760353@qq.com)
|
| 提交单位 |
Ningbo University |
| 提交日期 |
2024-12-03 |
