| 项目编号 |
PRJCA033478 |
| 项目标题 |
Metabolic Recoding of 5'-Methylthioadenosine- Mediated H3K79me1 Demethylation Antagonizes Cadmium-Induced Breast Cancer Progression by Maintaining Autophagic Flux-3 |
| 涉及领域 |
Medical |
| 数据类型 |
Proteome
|
| 物种名称 |
|
| 描述信息 |
The samples were subsequently loaded onto a 12% separation gel and subjected to SDS-PAGE electrophoresis for protein separation. The target band was excised into 1 mm³ gel pieces, which were then digested with trypsin. An extraction solution was prepared consisting of 5% trifluoroacetic acid (TFA), 50% acetonitrile (ACN), and 45% water to extract the peptides. Desalting was performed using a self-packed desalting column, followed by solvent evaporation in a vacuum centrifugal concentrator at 45°C to prepare for subsequent mass spectrometry analysis. Finally, the peptides were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with an Acclaim PepMap RPLC C18 column. The mobile phase consisted of water containing 0.1% formic acid and an 80% ACN solution, with a flow rate of 300 nL/min and an analysis duration of 120 minutes. PEAKS Studio software was employed to search target protein databases and identify variable modifications. Monomethylation of lysine (K)/ arginine (R)(+14.02 Da), dimethylation of K/R(+28.03 Da), and trimethylation of K (+42.05 Da) were specified as methylation modifications. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2026-01-15 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 40919610
|
5’‐Methylthioadenosine Metabolic Reprogramming Drives H3K79 Monomethylation‐Mediated PAK2 Upregulation to Promote Cadmium‐Induced Breast Cancer Progression by Impairing Autophagic Flux
|
Advanced Science
|
10.1002/advs.202500941
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China
|
|
82173473
|
|
| National Natural Science Foundation of China
|
|
82173468
|
|
|
|
| 提交者 |
Jingdian
li (lijingdian_tmmu@163.com)
|
| 提交单位 |
Third Military Medical University(Army Medical University) |
| 提交日期 |
2024-12-10 |
