| 描述信息 |
The aim of this project is to explore the potential application of batch RNA sequencing technology in clinical routine of Philadelphia chromosome negative myeloproliferative neoplasms(MPNs) for better patient management . Faced with the high cost and complexity of advanced technologies such as single-cell RNA sequencing and mass cytometry analysis, this project proposes bulk RNA sequencing as a cost-effective and easier to apply alternative method in clinical practice. The study analyzed peripheral blood and bone marrow samples of MPNs patients in the early stages of treatment (including polycythemia vera PV, essential thrombocytosis ET, and primary myelofibrosis PMF), combined with microarray datasets [GSE26049, GSE2191], and used bioinformatics methods to decipher gene mutations and immune profiles. The research results indicate that bulk RNA sequencing is a tool suitable for routine clinical practice, providing comprehensive insights into the immune and genetic profiles of MPNs. This method is expected for personalized treatment strategies, improving prognostic accuracy, and ultimately helping better MPNs patients management. The research team consists of Shenglong Li, Sanyun Wu, Mingli Xu, Xuedong Li, Xuelan Zuo, and Yingying Wang. |
