Accession |
PRJCA033976 |
Title |
2019YFA0802001-transcription-factor-induced transdifferentiation from fibroblasts to hepatocytes ATAC sequencing |
Relevance |
biology |
Data types |
Epigenomics
|
Organisms |
Mus musculus
|
Description |
Nuclei isolated from 50,000 counted cells were used for transposition reaction with Tn5 transposase (Vazyme Biotech). 10 PCR cycles were performed for library setup for each reaction mix using high-fidelity 2x PCR Master Mix (New England Biolabs) using primers with unique barcodes (Vazyme Biotech). Pair-end sequencing (150bp x 2) was performed on Illumina HiSeq X Ten instrument (CloudHealth Genomics Ltd.) |
Sample scope |
Multiisolate |
Release date |
2024-12-12 |
Grants |
Agency |
program |
Grant ID |
Grant title |
Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2019YFA0802001
|
|
|
Submitter |
Lijian
Hui (huilab@sibcb.ac.cn)
|
Organization |
Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences |
Submission date |
2024-12-12 |