| 描述信息 |
RNA-seq libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA) following standard protocols. Poly(A)-containing transcripts were enriched with Oligo(dT)-coated magnetic beads from total RNA. For each sample, adapters with unique barcodes were ligated to the end-polished cDNA fragments. The libraries were amplified by PCR and quantitated by Qubit 2.0 (Thermo Scientific, Waltham, MA). RNA libraries were sequenced on the Illumina HiSeq 4000 platform. RNA quantity and integrity were determined using NanoDrop 2000 (Thermo Scientific, Waltham, MA) and Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). |
