| 项目编号 | PRJCA034670 | ||||||||||||||||||||
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| 项目标题 | Exploring maize transcriptional regulatory landscape through large-scale profiling of transcription factor binding sites | ||||||||||||||||||||
| 涉及领域 | Agricultural | ||||||||||||||||||||
| 数据类型 | Raw sequence reads | ||||||||||||||||||||
| 物种名称 | Zea mays | ||||||||||||||||||||
| 描述信息 | Understanding gene regulatory networks (GRNs) is essential for improving maize yield and quality through molecular breeding approaches. The lack of comprehensive transcription factor (TF)-DNA interaction data has hindered accurate GRN predictions, limiting our insight into the regulatory mechanisms. Here, we performed large-scale profiling of maize TF binding sites. We obtained and collected reliable binding profiles for 513 TFs, identified 394,136 binding sites, and constructed an accuracy-enhanced maize GRN (mGRN+) by integrating chromatin accessibility and gene expression data. The mGRN+ comprises 397,699 regulatory relationships. We further divided the mGRN+ into multiple modules across six major tissues. Using machine learning algorithms, we optimized the mGRN+ to improve prediction accuracy of gene functions and key regulators. Through independent genetic validation experiments, we further confirmed the reliability of these predictions. This work provides the largest collection of experimental TF binding sites in maize and a highly optimized regulatory network, both of which can serve as valuable resources for the study of maize gene function and for crop improvement applications. | ||||||||||||||||||||
| 样品范围 | Multiisolate | ||||||||||||||||||||
| 发布日期 | 2025-08-07 | ||||||||||||||||||||
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| 提交者 | Qiang Huo (huoqiang@cau.edu.cn) | ||||||||||||||||||||
| 提交单位 | China Agricultural University | ||||||||||||||||||||
| 提交日期 | 2024-11-28 |