Accession PRJCA034711
Title Fine Mapping and Candidate Gene Analysis of Gynoecious Gene Gy1-1 In Bitter Gourd
Relevance Agricultural
Data types Genome sequencing
Organisms Momordica charantia
Description To investigate the function and molecular mechanism of the gynoecious genes in bitter gourd, the gynoecious inbred line gy1 was used to hybridize with the monoecious inbred lines MC52-1 and k8 to construct F2 segregating populations for genetic analysis of gynoecious genes. The results showed that the gynoecious traits were controlled by a single nuclear recessive gene, which was named gy1-1. The F2 segregating population obtained from hybridization of gynoecious line gy1 and monoecious line K8 were applied to construct extreme trait mixed pools of gynoecious line and monoecious line (female flower rate<3%). Through whole genome resequencing (BSA-seq) analysis combined with map-based cloning technology, the gynoecious gene gy1-1 was found to be mapped within the interval of marked G-10 and G-12. The related interval is located at 20.98-21.05 Mb on chromosome MC01, with a size of about 70.97 kb.
Sample scope Multiisolate
Release date 2025-01-08
Grants
Agency program Grant ID Grant title
Natural Science Foundation project from Guangxi 2018GXNSFBA281154
Submitter e.g.yuhui e.g.huang (34955959@qq.com)
Organization Guangxi Academy of Agricultural Sciences
Submission date 2025-01-07

Project Data

Resource name Description
BioSample (4) -
SAMC4537012 BSA offspring mixing pool( monoecious )
SAMC4537011 BSA offspring mixing pool( gynoecious )
SAMC4537010 male parent(the monoecious inbred lines k8)
SAMC4537009 female parent(the gynoecious inbred line gy1)
GSA (1) -
CRA022047 Bitter gourd High-throughput genotyping by whole-genome resequencing