| 项目编号 | PRJCA035268 | ||||||||
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| 项目标题 | Marmota metagenome Genome sequencing | ||||||||
| 涉及领域 | Medical | ||||||||
| 数据类型 | Metagenome | ||||||||
| 物种名称 | gut metagenome | ||||||||
| 描述信息 | For viral metagenomic analysis, 70 wild adult marmots were captured in Qinghai Province, China, in 2023. Samples of each marmot's intestinal contents were collected and individually banked to create a library of 70 marmot intestinal content samples. The marmota intestinal content samples were ground using a cryomill, resuspended in 1 mL of DPBS, and quickly frozen at -80°C. The samples were then thawed and ground three times. After centrifugation (10 min, 15,000 g), the supernatant was collected. This supernatant was filtered through a 0.45 mm filter (Millipore) to remove particles the size of eukaryotic and bacterial cells. Subsequently, 200 uL of supernatant from each pool was treated with a nuclease mixture to reduce the concentration of free (non-viral-encapsulated) nucleic acids. The remaining total nucleic acids were isolated using the QIAamp MinElute Virus Spin Kit according to the manufacturer's protocol. Seventy libraries were constructed using the Nextera XT DNA Sample Preparation Kit (Illumina), and 250 base pair sequencing was performed on each library using the MiSeq Illumina platform. | ||||||||
| 样品范围 | Multispecies | ||||||||
| 发布日期 | 2025-01-19 | ||||||||
| 项目资金来源 |
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| 提交者 | Xiaojie Jiang (j2521555180@outlook.com) | ||||||||
| 提交单位 | Wuxi Blood Center | ||||||||
| 提交日期 | 2025-01-19 |