项目编号

PRJCA035434

项目标题

The regulatory role of the circadian clock in metabolism and homeostasis of tissues and organs

涉及领域

Medical

数据类型

Transcriptome or Gene expression

物种名称

Mus musculus
Danio rerio

描述信息

This project investigated the coupling between the circadian clock and metabolism under physiological conditions and the underlying mechanism of maintaining homeostasis of tissues and organs, the mechanism of circadian misalignment leading to metabolic disorders and metabolic diseases of important tissues and organs, and establish methods for employing metabolites to interveneor modulate circadian clock disorders. We used the model organisms fruitflies, zebrafish and mice, as well as using "gene trap-based tissue-specific reconstitution technology" to establish new animal models of tissue-specific reconstitution and recombination reconstitution, zebrafish high-throughput metabolite screening technology, and multi-omics analysis and other technologies: (1) to screen, identify and study the key genes and metabolites of hierarchical coupling and parallel coupling between the circadian clock and metabolism in important tissues and organs; to elucidate the circadian mechanism underlying the coupling and homeostasis maintenance of the circadian clock and metabolism in important tissues and organs, and conduct cross-species comparisons of the circadian clock and metabolism coupling and homeostasis maintenance mechanisms; (2) to use multi-omics technologies to analyze the circadian clock genes of animals including fruitflies, zebrafish and mice with abnormal circadian clocks; (3) Using new tissue-specific reconstruction models (such as Bmal1/bmal1a/cycle) and related knockout models, as well as three types of model animals induced by light exposure, abnormal feeding, etc., to determine the changes in genes, proteins and metabolites of important tissues and organs, including the brain, liver, intestine and pancreas, and to identify the health status of experimental animals through key physiological and pathological indicators, in order to study the pathogenesis of metabolic disorders caused by abnormal circadian clock at the molecular and cellular levels; (4) Using transgenic zebrafish containing luciferase driven by E-box, RRE-box or D-box-containing promoters, and zebrafish induced by light exposure and abnormal feeding in the background of important circadian clock gene mutations, to screen approximately 10,000 metabolites/compounds to identify metabolites that can change the period, phase or amplitude of the circadian clock or intervene/adjust circadian clock abnormalities; use fruitflies, zebrafish and mice to study the methods and mechanisms of metabolites to interveneor modulate circadian clock abnormalities; to study the mechanism of action of adenosine and metformin on the circadian clocks of nematodes, fruit flies, zebrafish, and mice, and use multi-omics and other technologies to analyze the oscillation patterns of adenosine and its related metabolic enzymes in the brain, liver, intestine, etc. of four model animals, as well as the patterns and mechanisms of changes in the transcriptome, proteome, and metabolome of three model animals treated with adenosine, adenosine receptor antagonists, and metformin; use blood samples from obese and diabetic patients and liver samples from corresponding mouse, zebrafish, and fruit fly metabolic disease models to conduct omics analysis to clarify new mechanisms of the effects of obesity and diabetes on the circadian clock. To this end, this project has set up three closely related research subprojects: Subproject 1, the coupling between the circadian clock and the metabolism of important tissues and organs and the mechanism of maintaining homeostasis; Subproject 2, the pathogenesis of metabolic disorders of important tissues and organs caused by circadian misalignment; Subproject 3, investigation of metabolite intervention for circadian clock disorders.

样品范围

Multiisolate

发布日期

2025-01-22

项目资金来源

机构	项目类型	授权项目ID	授权项目名称
Ministry of Science and Technology of the People's Republic of China (MOST)	National Key Technologies R&D Program	2019YFA0802400	The regulatory role of the circadian clock in metabolism and homeostasis of tissues and organs

提交者

Taole LIU (liutaole@suda.edu.cn)

提交单位

Soochow University

提交日期

2025-01-22

项目包含数据信息

资源名称	描述
BioSample (94) show	-
SAMC4601744	RNA-Seq of danio rerio: clock1a-/- testis RA12h
SAMC4601743	RNA-Seq of danio rerio: clock1a-/- testis veh12h
SAMC4601742	RNA-Seq of danio rerio: clock1a-/- testis RA6h
SAMC4601741	RNA-Seq of danio rerio: clock1a-/- testis veh6h
SAMC4601740	RNA-Seq of danio rerio: adult testis atRA 2h Replicate 3
SAMC4601739	RNA-Seq of danio rerio: adult testis atRA 2h Replicate 2
SAMC4601738	RNA-Seq of danio rerio: adult testis atRA 2h Replicate 1
SAMC4601737	RNA-Seq of danio rerio: adult testis vehicle 2h Replicate 3
SAMC4601736	RNA-Seq of danio rerio: adult testis vehicle 2h Replicate 2
SAMC4601735	RNA-Seq of danio rerio: adult testis vehicle 2h Replicate 1
SAMC4601734	RNA-Seq of danio rerio: adult testis ZT20 D2 Replicate 2
SAMC4601733	RNA-Seq of danio rerio: adult testis ZT16 D2 Replicate 2
SAMC4601732	RNA-Seq of danio rerio: adult testis ZT12 D2 Replicate 2
SAMC4601731	RNA-Seq of danio rerio: adult testis ZT8 D2 Replicate 2
SAMC4601730	RNA-Seq of danio rerio: adult testis ZT4 D2 Replicate 2
SAMC4601729	RNA-Seq of danio rerio: adult testis ZT0 D2 Replicate 2
SAMC4601728	RNA-Seq of danio rerio: adult testis ZT20 D1 Replicate 2
SAMC4601727	RNA-Seq of danio rerio: adult testis ZT16 D1 Replicate 2
SAMC4601726	RNA-Seq of danio rerio: adult testis ZT12 D1 Replicate 2
SAMC4601725	RNA-Seq of danio rerio: adult testis ZT8 D1 Replicate 2
SAMC4601724	RNA-Seq of danio rerio: adult testis ZT4 D1 Replicate 2
SAMC4601723	RNA-Seq of danio rerio: adult testis ZT0 D1 Replicate 2
SAMC4601722	RNA-Seq of danio rerio: adult testis ZT20 D2 Replicate 1
SAMC4601721	RNA-Seq of danio rerio: adult testis ZT16 D2 Replicate 1
SAMC4601720	RNA-Seq of danio rerio: adult testis ZT12 D2 Replicate 1
SAMC4601719	RNA-Seq of danio rerio: adult testis ZT8 D2 Replicate 1
SAMC4601718	RNA-Seq of danio rerio: adult testis ZT4 D2 Replicate 1
SAMC4601717	RNA-Seq of danio rerio: adult testis ZT0 D2 Replicate 1
SAMC4601716	RNA-Seq of danio rerio: adult testis ZT20 D1 Replicate 1
SAMC4601715	RNA-Seq of danio rerio: adult testis ZT16 D1 Replicate 1
SAMC4601714	RNA-Seq of danio rerio: adult testis ZT12 D1 Replicate 1
SAMC4601713	RNA-Seq of danio rerio: adult testis ZT8 D1 Replicate 1
SAMC4601712	RNA-Seq of danio rerio: adult testis ZT4 D1 Replicate 1
SAMC4601711	RNA-Seq of danio rerio: adult testis ZT0 D1 Replicate 1
SAMC4601710	RNA-Seq of mus musculus: adult testis ZT20 desynchrony Replicate 3
SAMC4601709	RNA-Seq of mus musculus: adult testis ZT20 desynchrony Replicate 2
SAMC4601708	RNA-Seq of mus musculus: adult testis ZT20 desynchrony Replicate 1
SAMC4601707	RNA-Seq of mus musculus: adult testis ZT16 desynchrony Replicate 3
SAMC4601706	RNA-Seq of mus musculus: adult testis ZT16 desynchrony Replicate 2
SAMC4601705	RNA-Seq of mus musculus: adult testis ZT16 desynchrony Replicate 1
SAMC4601704	RNA-Seq of mus musculus: adult testis ZT12 desynchrony Replicate 3
SAMC4601703	RNA-Seq of mus musculus: adult testis ZT12 desynchrony Replicate 2
SAMC4601702	RNA-Seq of mus musculus: adult testis ZT12 desynchrony Replicate 1
SAMC4601701	RNA-Seq of mus musculus: adult testis ZT8 desynchrony Replicate 3
SAMC4601700	RNA-Seq of mus musculus: adult testis ZT8 desynchrony Replicate 2
SAMC4601699	RNA-Seq of mus musculus: adult testis ZT8 control Replicate 1
SAMC4601698	RNA-Seq of mus musculus: adult testis ZT4 control Replicate 3
SAMC4601697	RNA-Seq of mus musculus: adult testis ZT4 desynchrony Replicate 2
SAMC4601696	RNA-Seq of mus musculus: adult testis ZT4 desynchrony Replicate 1
SAMC4601695	RNA-Seq of mus musculus: adult testis ZT0 desynchrony Replicate 3
SAMC4601694	RNA-Seq of mus musculus: adult testis ZT0 desynchrony Replicate 2
SAMC4601693	RNA-Seq of mus musculus: adult testis ZT0 desynchrony Replicate 1
SAMC4601692	RNA-Seq of mus musculus: adult testis ZT20 control Replicate 3
SAMC4601691	RNA-Seq of mus musculus: adult testis ZT20 control Replicate 2
SAMC4601690	RNA-Seq of mus musculus: adult testis ZT20 control Replicate 1
SAMC4601689	RNA-Seq of mus musculus: adult testis ZT16 control Replicate 3
SAMC4601688	RNA-Seq of mus musculus: adult testis ZT16 control Replicate 2
SAMC4601687	RNA-Seq of mus musculus: adult testis ZT16 control Replicate 1
SAMC4601686	RNA-Seq of mus musculus: adult testis ZT12 control Replicate 3
SAMC4601685	RNA-Seq of mus musculus: adult testis ZT12 control Replicate 2
SAMC4601684	RNA-Seq of mus musculus: adult testis ZT12 control Replicate 1
SAMC4601683	RNA-Seq of mus musculus: adult testis ZT8 control Replicate 3
SAMC4601682	RNA-Seq of mus musculus: adult testis ZT8 control Replicate 2
SAMC4601681	RNA-Seq of mus musculus: adult testis ZT8 control Replicate 1
SAMC4601680	RNA-Seq of mus musculus: adult testis ZT4 control Replicate 3
SAMC4601679	RNA-Seq of mus musculus: adult testis ZT4 control Replicate 2
SAMC4601678	RNA-Seq of mus musculus: adult testis ZT4 control Replicate 1
SAMC4601677	RNA-Seq of mus musculus: adult testis ZT0 control Replicate 3
SAMC4601676	RNA-Seq of mus musculus: adult testis ZT0 control Replicate 2
SAMC4601675	RNA-Seq of mus musculus: adult testis ZT0 control Replicate 1
SAMC4601674	RNA-Seq of mus musculus: adult testis ZT20 D2 Replicate 2
SAMC4601673	RNA-Seq of mus musculus: adult testis ZT20 D2 Replicate 1
SAMC4601672	RNA-Seq of mus musculus: adult testis ZT16 D2 Replicate 2
SAMC4601671	RNA-Seq of mus musculus: adult testis ZT16 D2 Replicate 1
SAMC4601670	RNA-Seq of mus musculus: adult testis ZT12 D2 Replicate 2
SAMC4601669	RNA-Seq of mus musculus: adult testis ZT12 D2 Replicate 1
SAMC4601668	RNA-Seq of mus musculus: adult testis ZT8 D2 Replicate 2
SAMC4601667	RNA-Seq of mus musculus: adult testis ZT8 D2 Replicate 1
SAMC4601666	RNA-Seq of mus musculus: adult testis ZT4 D2 Replicate 2
SAMC4601665	RNA-Seq of mus musculus: adult testis ZT4 D2 Replicate 1
SAMC4601664	RNA-Seq of mus musculus: adult testis ZT0 D2 Replicate 2
SAMC4601663	RNA-Seq of mus musculus: adult testis ZT0 D2 Replicate 1
SAMC4601662	RNA-Seq of mus musculus: adult testis ZT20 D1 Replicate 2
SAMC4601661	RNA-Seq of mus musculus: adult testis ZT20 D1 Replicate 1
SAMC4601660	RNA-Seq of mus musculus: adult testis ZT16 D1 Replicate 2
SAMC4601659	RNA-Seq of mus musculus: adult testis ZT16 D1 Replicate 1
SAMC4601658	RNA-Seq of mus musculus: adult testis ZT12 D1 Replicate 2
SAMC4601657	RNA-Seq of mus musculus: adult testis ZT12 D1 Replicate 1
SAMC4601656	RNA-Seq of mus musculus: adult testis ZT8 D1 Replicate 2
SAMC4601655	RNA-Seq of mus musculus: adult testis ZT8 D1 Replicate 1
SAMC4601654	RNA-Seq of mus musculus: adult testis ZT4 D1 Replicate 2
SAMC4601653	RNA-Seq of mus musculus: adult testis ZT4 D1 Replicate 1
SAMC4601652	RNA-Seq of mus musculus: adult testis ZT0 D1 Replicate 2
SAMC4601651	RNA-Seq of mus musculus: adult testis ZT0 D1 Replicate 1
GSA (1)	-
CRA022478	circadian testis transcriptome