| 项目编号 |
PRJCA036032 |
| 项目标题 |
Spatiotemporal Dynamics of Neuron Differentiation and Migration in the Developing Human Spinal Cord |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Mus musculus
Homo sapiens
|
| 描述信息 |
In this study, we performed single-cell transcriptome sequencing of human spinal cord from Carnegie Stage (CS) 16-21 and mouse spinal cord from embryonic day (E) 8.0-11.5, complemented by in situ sequencing of human spinal cord (CS 16-20). Our results reveal the critical role of the precursor state in neuronal differentiation and migration, identifying key transcription factors that regulate these processes across species. Notably, each neuronal lineage expresses unique markers as early as the progenitor stage at the spinal cord midline, and later undergoes a shared transcriptional program during precursor commitment that guides migration. This synchronized migration, validated by spatial transcriptomics, occurs in both dorsal and ventral regions. Our findings offer new insights into the migration patterns and regulatory factors that guide spinal cord neuron subtype specification during embryogenesis, deepening our understanding of neural circuit formation in the spinal cord. |
| 样品范围 |
Single cell |
| 发布日期 |
2025-09-18 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 40825501
|
Spatiotemporal dynamics of neuron differentiation and migration in the developing human spinal cord
|
Journal of Genetics and Genomics = Yi Chuan Xue Bao
|
10.1016/j.jgg.2025.08.004
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
General Program
|
32225012
|
|
| National Key R&D Program of China
|
Key Program
|
2024YFA1802300
|
|
| National Key R&D Program of China
|
Key Program
|
2023YFF1204701
|
|
| Guangzhou National Laboratory
|
Major Project
|
GZNL2023A02005
|
|
| Pearl River Talent Recruitment Program
|
|
2021ZT09Y233
|
|
| National Natural Science Foundation of China (NSFC)
|
General Program
|
32200662
|
|
|
|
| 提交者 |
Jiekai
Chen (chen_jiekai@gibh.ac.cn)
|
| 提交单位 |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| 提交日期 |
2025-01-27 |
