| 项目编号 |
PRJCA036439 |
| 项目标题 |
The alterations in gene expression and alternative splicing induced by the plasmid-mediated overexpression of GFP and P2RY12 within the A549 cell line |
| 涉及领域 |
Medical |
| 数据类型 |
Raw sequence reads
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Genetic studies frequently observe phenotypic modifications and their effects on cellular functions through the upregulation of target gene expression, but the unique roles of the cell line and its introduced plasmids in influencing these functions have not been entirely uncovered. In this research, we developed two distinct cell lines derived from the A549 cell line: one that stably overexpresses GFP and another that is a polyclonal stable line overexpressing both GFP and P2RY12. We then utilized transcriptome sequencing technology to screen out differentially expressed genes and genes with Differential Transcript Usage after GFP over-expression and P2RY12 over-expression. We found that comparing with A549, more than seventeen hundred genes were differentially expressed in both GFP-OE and P2RY12-OE cells, while only eight hundred and sixty-six DEGs were identified between GFP-OE and P2RY12-OE cells. Notably, the differences in transcript usage were relatively minor, with only over four hundred genes exhibiting changes across all three groups. Functional analysis of DEGs and gDTUs showed that they both were highly enriched in the pathways associated with cell proliferation and migration. In summary, we made an extensive analysis of the transcriptome profile of gene expression and alternative splicing by GFP-OE and P2RY12-OE, enhancing our comprehension of how genes function within cells and the processes that control gene expression. |
| 样品范围 |
Synthetic |
| 发布日期 |
2025-02-21 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 40243586
|
Alterations in Gene Expression and Alternative Splicing Induced by Plasmid-Mediated Overexpression of GFP and P2RY12 Within the A549 Cell Line
|
International Journal of Molecular Sciences
|
10.3390/ijms26072973
|
2025
|
| 41516360
|
Long-Read Sequencing Reveals Cell- and State-Specific Alternative Splicing in 293T and A549 Cell Transcriptomes
|
International Journal of Molecular Sciences
|
10.3390/ijms27010487
|
2026
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| NSFSC
|
|
2024NSFSC1978
|
Key project of Sichuan Science and Technology Education Joint Fund
|
|
|
| 提交者 |
liang
Leng (lling@cdutcm.edu.cn)
|
| 提交单位 |
Chengdu University of Chinese Medicine |
| 提交日期 |
2025-02-20 |
