| 项目编号 | PRJCA036988 | ||||||||||
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| 项目标题 | NEK8 blockade potentiates radiation-induced antitumor immunity and abscopal effect via lactate metabolism reprogramming in colorectal cancer | ||||||||||
| 涉及领域 | Medical | ||||||||||
| 数据类型 |
Epigenomics
Transcriptome or Gene expression Single cell sequencing Proteome Omics |
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| 物种名称 |
Mus musculus
Homo sapiens Mus |
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| 描述信息 | In this project, we established an intratumoral iteration model of colorectal cancer radiotherapy resistance, designated as IR-MC38, and compared its tumor microenvironment to that of the parental MC38 tumor using single-cell sequencing. Through RNA sequencing, we identified NEK8 as a key gene associated with the anti-tumor immunity of CD8+T cells. Subsequently, we performed comprehensive multi-omics analyses on NEK8, including proteomics, CUT&Tag sequencing, and bulk RNA sequencing, to elucidate its role and underlying mechanisms. | ||||||||||
| 样品范围 | Multiisolate | ||||||||||
| 发布日期 | 2025-05-24 | ||||||||||
| 出版信息 |
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| 项目资金来源 |
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| 提交者 | li liang (redsnow007@126.com) | ||||||||||
| 提交单位 | Southern Medical University | ||||||||||
| 提交日期 | 2025-03-06 |
| 资源名称 | 描述 |
|---|---|
| BioSample (22) show | - |
| GSA (4) | - |
| CRA023580 | scRNA-seq of the IR-resistant IR-MC38 tumors and IR-sensitive MC38 tumors |
| CRA023695 | RNA-seq of the IR-resistant IR-MC38 cells and IR-sensitive MC38 cells |
| CRA023632 | CUT&Tag of H3K8la in MC38-shNEK8 andMC38-shNC cells |
| CRA023578 | RNA seq of shNC_IR and shNEK8_IR tumors. |