| 项目编号 |
PRJCA037022 |
| 项目标题 |
Deciphering signaling mechanisms and developmental dynamics in extraembryonic mesoderm specification from hESCs |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads
Single cell sequencing
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Extraembryonic mesoderm (ExM) is crucial for human development, yet its specification is poorly understood. Human embryonic stem cell (hESC)-based models, including embryoids and differentiated derivatives, are emerging as promising tools for studying ExM development. Despite this, the signaling mechanisms and developmental dynamics that underlie ExM specification from hESCs remain challenging to study. Here, we demonstrate that the modulation of BMP, WNT, and Nodal signaling pathways can rapidly and efficiently induce the differentiation of both naive and primed hESCs into ExM-like cells (ExMs), predominantly through intermediates exhibiting a primitive streak (PS)-like phenotype. We reveal the regulatory roles of WNT and Nodal signaling in ExM specification from hESCs. Furthermore, we find that the initial pluripotent state influences hESC-based ExM specification, including signal response, cellular composition, developmental progression, and transcriptional characteristics of the resulting ExMs. Our study provides promising models for dissecting human ExM development and sheds light on the signaling principles and developmental dynamics underlying ExM specification from both naive and primed hESCs. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2025-04-07 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 40399284
|
Deciphering signaling mechanisms and developmental dynamics in extraembryonic mesoderm specification from hESCs
|
Nature Communications
|
10.1038/s41467-025-59491-x
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2022YFA1103100
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2020YFA0112700
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2021YFA0805700
|
|
| National Natural Science Foundation of China (NSFC)
|
Key Program
|
32130034
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32360177
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32260176
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32360178
|
|
| National Natural Science Foundation of China (NSFC)
|
General Program
|
32470859
|
|
| Yunnan Province Science and Technology Department
|
Major Basic Research Project of Science and Technology of Yunnan
|
202102AA100053
|
|
|
|
| 提交者 |
Yu
Yin (yiny@lpbr.cn)
|
| 提交单位 |
Institute of Primate Translational Medicine, Kunming University of Science and Technology |
| 提交日期 |
2025-03-07 |
