| 描述信息 |
In this study, we investigated the antitumor effects and potential mechanisms of Codonopsis pilosula in LSCC through network pharmacology, experimental validation and bioinformatics analysis. Firstly, effective compounds and targets of Codonopsis pilosula were screened by TCMSP, ETCM and BATMAN-TCM databases. In addition, 22 common targets related to LSCC were screened by combining DisGeNET, GeneCards databases and Cytoscape software. KEGG pathway enrichment analysis showed that Codonopsis pilosula-LSCC targets were mainly involved in HIF-1, TNF, IL-17 and FoxO signaling pathways. Based on TCGA and GEO database analysis, MAPK3 was identified as the core target of Codonopsis pilosula-LSCC. The molecular docking results showed that a variety of effective compounds from Codonopsis pilosula had strong binding abilities to MAPK3, among them, Caprylic Acid, Emodin and Luteolin have been confirmed by LC-MS. Subsequently, qPCR analysis indicated that MAPK3 was highly expressed in LSCC tissues. MAPK3 knockdown significantly inhibits LSCC cell proliferation, migration and invasion. It also suppresses LSCC cell growth by blocking the cell cycle and inducing apoptosis. In conclusion, Codonopsis pilosula exerts antitumor effects in LSCC through the regulation of multiple signaling pathways and provides a theoretical basis for its clinical application. |
