| 项目编号 |
PRJCA040216 |
| 项目标题 |
The Specificity Landscape of AtWRKYs Reveals the Bidirectional Influence of DNA Methylation |
| 涉及领域 |
Agricultural |
| 数据类型 |
Epigenomics
Raw sequence reads
Genome sequencing
|
| 物种名称 |
Arabidopsis thaliana
|
| 描述信息 |
A distinct epigenetic feature of plants is that DNA methylation can occur in non-CG contexts. While non-CG methylation is involved in plant-specific mechanisms of transcriptional regulation, their roles in TF-DNA interactions are yet to be systematically examined. WRKY-family TFs are plant-specific and primarily recognize non-CG cytosines, here we investigated how their specificity and genome-wide cis-regulatory elements (CREs) are affected by DNA methylations in all contexts. By generating 468 SELEX/DAP-seq libraries for 54 AtWRKYs, we show that DNA methylation changes the specificity for both the monomeric and dimeric binding of WRKYs, and has overall increased the specificity difference between WRKYs. We curated 236 WRKY motifs and clustered them into 11 classes, of which 5 classes represent unreported novel specificities of WRKYs. |
| 样品范围 |
Synthetic |
| 发布日期 |
2025-10-07 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 41261862
|
The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylation
|
Nucleic Acids Research
|
10.1093/nar/gkaf1168
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
General Program
|
32370582
|
|
|
|
| 项目相关网站链接 |
|
| 提交者 |
Dingkun
Jiang (jiangdk0722@163.com)
|
| 提交单位 |
Fujian Agriculture and Forestry University |
| 提交日期 |
2025-05-06 |
