| Accession |
PRJCA040216 |
| Title |
The Specificity Landscape of AtWRKYs Reveals the Bidirectional Influence of DNA Methylation |
| Relevance |
Agricultural |
| Data types |
Epigenomics
Raw sequence reads
Genome sequencing
|
| Organisms |
Arabidopsis thaliana
|
| Description |
A distinct epigenetic feature of plants is that DNA methylation can occur in non-CG contexts. While non-CG methylation is involved in plant-specific mechanisms of transcriptional regulation, their roles in TF-DNA interactions are yet to be systematically examined. WRKY-family TFs are plant-specific and primarily recognize non-CG cytosines, here we investigated how their specificity and genome-wide cis-regulatory elements (CREs) are affected by DNA methylations in all contexts. By generating 468 SELEX/DAP-seq libraries for 54 AtWRKYs, we show that DNA methylation changes the specificity for both the monomeric and dimeric binding of WRKYs, and has overall increased the specificity difference between WRKYs. We curated 236 WRKY motifs and clustered them into 11 classes, of which 5 classes represent unreported novel specificities of WRKYs. |
| Sample scope |
Synthetic |
| Release date |
2025-10-07 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
General Program
|
32370582
|
|
|
|
| External link |
|
| Submitter |
Dingkun
Jiang (jiangdk0722@163.com)
|
| Organization |
Fujian Agriculture and Forestry University |
| Submission date |
2025-05-06 |
