| 项目编号 |
PRJCA040908 |
| 项目标题 |
The Role of METTL3 in Transposable Elements Regulation and 2C-like Program Induction in Mouse Embryonic Stem Cell |
| 涉及领域 |
Epigenetics and Development |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
METTL3 is a crucial mRNA methyltransferase in mammals, essential for the regulation of gene expression and various biological processes. Here, we demonstrate that Mettl3 knockout (KO) in mouse embryonic stem cells (mESCs) leads to the up-regulation of most transposable elements (TEs) and upregulation of 2-cell (2C)-like genes in a m6A enzyme activity-dependent manner, independent of culture conditions. Furthermore, embryo chimera experiments using a transient METTL3 degradation system (dTAG) revealed that METTL3-deficient mESCs can contribute to trophectoderm lineages at the blastocyst stage, indicative of expanded developmental potential. These findings highlight the role of METTL3-mediated m6A modifications in regulating the transcriptional and developmental plasticity of mESCs and suggest a link between m6A loss and the acquisition of a 2C-like state with features of extended potency. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2025-12-03 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 41264067
|
The role of METTL3 in transposable elements regulation and 2C-like program induction in mouse embryonic stem cell
|
Cell Regeneration
|
10.1186/s13619-025-00262-w
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Key Research and Development Program of China
|
|
2024YFA1107000
|
|
| National Key Research and Development Program of China
|
|
2024YFA1107901
|
|
| National Key Research and Development Program of China
|
|
2021YFA1102200
|
|
| National Key Research and Development Program of China
|
|
2024YFA1802300
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32225012
|
|
| Science and Technology Planning Project of Guangdong Province
|
|
2023B1212060050
|
|
| Science and Technology Planning Project of Guangdong Province
|
|
2023B1212120009
|
|
|
|
| 提交者 |
Jiekai
Chen (chen_jiekai@gibh.ac.cn)
|
| 提交单位 |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| 提交日期 |
2025-05-29 |
