| 项目编号 |
PRJCA040939 |
| 项目标题 |
Single-cell and Spatial Transcriptomics Reveal Radiation-Induced Disruption of the Blood-Cerebrospinal Fluid Barrier |
| 涉及领域 |
Environmental |
| 数据类型 |
Single cell sequencing
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
Radiotherapy is an effective treatment for brain tumors but often leads to radiation-induced brain injury (RIBI) and even irreversible cognitive impairments. However, the pathophysiology underlying RIBI remains elusive, which hinders the development of effective therapies to mitigate its long-term consequences . Here, we employed unbiased single-cell RNA sequencing and spatial transcriptomics techniques to intricately characterize the acute, early delayed, and late phases of RIBI. Our findings shed light on the critical role of the blood-cerebrospinal fluid barrier (BCSFB) in RIBI progression. Increased permeability of the BCSFB facilitates the infiltration of immune cells into the brain parenchyma during the early delayed phase of RIBI. This infiltration initiates dynamic interactions with interferon-responsive microglia, triggering a cascade of inflammatory responses. Additionally, we identified a subtype of choroid plexus epithelial cells potentially involved in maintaining the integrity of the BCSFB. |
| 样品范围 |
Single cell |
| 发布日期 |
2025-05-31 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
General Program
|
32200511
|
|
| Beijing Natural Science Foundation
|
General Program
|
5242025
|
|
|
|
| 提交者 |
Gangqiao
Zhou (zhougq114@126.com)
|
| 提交单位 |
State Key Laboratory of Medical Proteomics, National Center for Protein Sciences, Beijing Institute of Radiation Medicine |
| 提交日期 |
2025-05-31 |
