| Accession | PRJCA041788 | ||||||||||||||||
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| Title | Midgut cell atlas of four important mosquito species and the key factors associated with DENV infection | ||||||||||||||||
| Relevance | Medical | ||||||||||||||||
| Data types |
Transcriptome or Gene expression
Single cell sequencing |
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| Organisms |
Aedes albopictus
Culex tritaeniorhynchus Aedes aegypti mixed sample Culex pipiens pallens |
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| Description | To establish midgut cell atlas of four medically important mosquitoes including Aedes aegypti, Aedes albopictus, Culex pipiens pallens and Culex tritaeniorhynchus, we developed and applied a compatible microwell-based scRNA-seq protocol to the female mosquitoes. We performed scRNA-seq experiments on the midgut samples of the four mosquito species at 7 days with only glucose solution to establish their midgut cell atlas. To decipher the transcriptional changes following the uninfectious bloodmeal, we utilized scRNA-seq experiments on both Aedes aegypti and Culex tritaeniorhynchus, respectively. Further, to characterize the dynamics of mosquito midgut post-infection, we performed scRNA-seq experiments on Aedes aegypti by infectious bloodmeal using DENV. | ||||||||||||||||
| Sample scope | Multispecies | ||||||||||||||||
| Release date | 2025-08-29 | ||||||||||||||||
| Publication |
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| Grants |
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| Submitter | Han Xia (hanxia@wh.iov.cn) | ||||||||||||||||
| Organization | Wuhan Institute of Virology, Chinese Academy of Sciences | ||||||||||||||||
| Submission date | 2025-06-13 |
| Resource name | Description |
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