| 项目编号 | PRJCA041788 | ||||||||||||||||
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| 项目标题 | Midgut cell atlas of four important mosquito species and the key factors associated with DENV infection | ||||||||||||||||
| 涉及领域 | Medical | ||||||||||||||||
| 数据类型 |
Transcriptome or Gene expression
Single cell sequencing |
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| 物种名称 |
Aedes albopictus
Culex tritaeniorhynchus Aedes aegypti mixed sample Culex pipiens pallens |
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| 描述信息 | To establish midgut cell atlas of four medically important mosquitoes including Aedes aegypti, Aedes albopictus, Culex pipiens pallens and Culex tritaeniorhynchus, we developed and applied a compatible microwell-based scRNA-seq protocol to the female mosquitoes. We performed scRNA-seq experiments on the midgut samples of the four mosquito species at 7 days with only glucose solution to establish their midgut cell atlas. To decipher the transcriptional changes following the uninfectious bloodmeal, we utilized scRNA-seq experiments on both Aedes aegypti and Culex tritaeniorhynchus, respectively. Further, to characterize the dynamics of mosquito midgut post-infection, we performed scRNA-seq experiments on Aedes aegypti by infectious bloodmeal using DENV. | ||||||||||||||||
| 样品范围 | Multispecies | ||||||||||||||||
| 发布日期 | 2026-01-25 | ||||||||||||||||
| 出版信息 |
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| 项目资金来源 |
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| 提交者 | Han Xia (hanxia@wh.iov.cn) | ||||||||||||||||
| 提交单位 | Wuhan Institute of Virology, Chinese Academy of Sciences | ||||||||||||||||
| 提交日期 | 2025-06-13 |