| 项目编号 |
PRJCA042760 |
| 项目标题 |
The function and polarization mechanism of cancer associated fibroblasts in liver cancer |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
Single cell sequencing
Proteome
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
We employed single-cell-resolution spatial multi-omics to comprehensively characterize the stromal architecture and immune microenvironment of HCC. Our study analyzed 135 tissue microarray (TMA) cores from 45 patients, spanning tumor regions, invasive margins, and adjacent normal tissue. We applied high-throughput CosMx spatial transcriptomics with both 1,000- and 6,000-gene panels, NanoString DSP spatial proteomics, and histopathological analysis via H&E staining. We also further integrated publicly available scRNA-seq datasets from immunotherapy-treated cohorts. By dissecting the synergistic effects of diverse cell types in the fibrotic niches at single-cell and spatial resolution, our study provides spatial mechanistic insights into how the endothelial-CAF-immune axis mediates HCC progression and the resistance to immunotherapy and targeted therapy, offering potential biomarkers for patient stratification and therapeutic targeting. |
| 样品范围 |
Single cell |
| 发布日期 |
2025-07-11 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
82090050
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82090052
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82090053
|
|
| Tsinghua University Initiative Scientific Research Program of Precision Medicine
|
|
2022ZLA007
|
|
| CAMS Innovation Fund for Medical Sciences
|
|
2019-I2M-5-056
|
|
|
|
| 提交者 |
Shizhong
Yang (ysza02008@btch.edu.cn)
|
| 提交单位 |
Beijing Tsinghua Chang gung Hospital |
| 提交日期 |
2025-07-07 |
