| 项目编号 |
PRJCA043826 |
| 项目标题 |
DDX5 orchestrates RNA homeostasis to ensure oocyte developmental competence |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
Genome sequencing
|
| 物种名称 |
|
| 描述信息 |
Oocyte development requires tight regulation of transcription and RNA metabolism, coordinated by RNA-binding proteins (RBPs) whose roles in mammalian oogenesis remain incompletely understood. Here, we identify the DEAD-box RNA helicase DDX5 as a key regulator of RNA homeostasis in oocytes. Oocyte-specific deletion of DDX5 leads to female sterility, marked by defective chromatin remodeling, meiotic arrest, increased aneuploidy, and fertilization failure. Mechanistically, DDX5 maintains RNA homeostasis through three interconnected processes: (1) promoting transcription via interaction with RNA Polymerase II in non-surrounded nucleolus (NSN)-stage germinal vesicle (GV) oocytes; (2) clearing retrotransposon RNAs to safeguard transcriptome integrity; and (3) supporting maternal mRNA storage by coordinating nuclear export, mitochondrial organization, and mitochondria-associated ribonucleoprotein domains (MARDO) assembly. Our study establishes DDX5 as a master regulator integrating transcriptional and post-transcriptional programs to ensure oocyte competence and fertility. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2026-01-11 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
32270856
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32270858
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32488101
|
|
| National Key Research and Development Program of China
|
|
2021YFA1100300
|
|
|
|
| 提交者 |
Jiayu
Chen (chenjiayu@tongji.edu.cn)
|
| 提交单位 |
Shanghai First Maternity and Infant Hospital |
| 提交日期 |
2025-07-27 |
