| 项目编号 |
PRJCA044109 |
| 项目标题 |
Riboregulation controls FXR1 condensates for mRNA localized translation and transport |
| 涉及领域 |
Medical |
| 数据类型 |
Proteome
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Biomolecular condensates are found throughout a diversity of eukaryotic cell types and cellular compartments and are involved in a variety of cellular functions. A given protein generally forms functionally and compositionally heterogeneous condensates, but the underlying regulatory mechanisms are unknown. Here, we found that different RNA motifs can modulate the formation of heterogeneous mRNA-protein complex (mRNP) condensates via riboregulation. Fragile X-related 1 (FXR1), identified here as an mRNA transporter, assembles distinct localized subcellular mRNP condensates that are associated with the cytosolic accumulation of G-quadruplex-containing pluripotent mRNAs and the localized translation of nucleoporin mRNAs at nuclear pores. The diverse locations and functions of FXR1 condensates depend on the unique RNA-protein interaction modules of its two RNA binding domains, as well as the opposing effects of binding different RNA motifs on the affinity of FXR1 for nuclear pores. More importantly, a decline in FXR1 and nuclear pore activity hinders the nuclear export of transcribed RNA, thereby aiding human embryonic stem cells (hESCs) achieving fate transition. Preventing this decline would result in impaired hESC differentiation. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2025-08-22 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 41022782
|
Modular RNA interactions shape FXR1 condensates involved in mRNA localization and translation
|
Nature Communications
|
10.1038/s41467-025-63700-y
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
82122005
|
|
| National Natural Science Foundation of China (NSFC)
|
|
92268205
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82330007
|
|
| National Key Research and Development Program of China
|
|
2019YFA0801800
|
|
| National Key Research and Development Program of China
|
|
2021YFA0805703
|
|
| National Key Research and Development Program of China
|
|
2019YFA0802600
|
|
| CAMS Innovation Fund for Medical Sciences
|
|
2021-I2M-1-019
|
|
| Haihe Laboratory of Cell Ecosystem Innovation Fund
|
|
22HHXBSS00027
|
|
|
|
| 提交者 |
Zhongyang
Chen (15071468653@163.com)
|
| 提交单位 |
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College |
| 提交日期 |
2025-08-01 |
