| 项目编号 | PRJCA044688 | ||||||||||
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| 项目标题 | MAT2A promotes atherosclerotic plaque vulnerability by mediating epigenetic reprogramming of macrophages | ||||||||||
| 涉及领域 | Medical | ||||||||||
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads Metabonomics |
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| 物种名称 |
Mus musculus
Homo sapiens |
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| 描述信息 | In order to undertake a systematic investigation into the metabolism of monocytes in atherosclerosis, an untargeted metabolomic profiling of peripheral blood monocytes was performed from patients who were positive for thin-cap fibroatheroma (TCFA) (n=38) in comparison to patients who were negative for TCFA (n=38). The investigation was conducted using a method of mass spectrometry, with the objective of examining the methionine metabolism of monocytes from patients who were TCFA-positive, in comparison to patients who were TCFA-negative. This investigation was performed in both the discovery cohort (n=76) and the validation cohort (n=200).To assess alterations in MAT2A-mediated methionine metabolism within atherosclerosis, mass spectrometry was applied utilizing MAT2A conditional knockout mice, MAT2A specific inhibitor and low-methionine diet.To elucidate the underlying mechanism alleviating atherosclerosis mediated by MAT2A deficiency, the aortas (n = 6 separated pools of 3 mice each per group) were collected from MAT2ACKOApoE-/- and MAT2Afl/flApoE-/- mice fed the HFD for 16 weeks and digested for cell sorting in flow cytometry of plaque macrophages and then analysed using RNA-seq.In order to further elucidate the underlying mechanism mediated by MAT2A deficiency, RNA-seq was utilised to assess the gene expression profiles in bone marrow-derived macrophages (BMDMs) from MAT2ACKO mice (n=3) and control MAT2Afl/fl mice (n=3). | ||||||||||
| 样品范围 | Multiisolate | ||||||||||
| 发布日期 | 2025-10-02 | ||||||||||
| 出版信息 |
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| 项目资金来源 |
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| 提交者 | ping sun (sunpinghmu@163.com) | ||||||||||
| 提交单位 | Harbin Medical University | ||||||||||
| 提交日期 | 2025-08-14 |