| 描述信息 |
The abuse of antibiotics in aquaculture has led to environmental pollution and an increase in bacterial resistance. Probiotics not only have antibacterial effects but also can promote animal growth and improve immunity. As a biological control method, they have the potential to replace antibiotics. The LV149 Bacillus brevibrio with broad-spectrum resistance isolated from the intestinal tract of Litopenaeus vannamei by the research group in the early stage has the application prospect of being used as a probiotic for farmed shrimp. This study verified that Bacillus brevis has antibacterial activity against Vibrio broad-spectrum, and analyzed the antibacterial mechanism of LV149 through the second-generation Illumina and third-generation PacBio genomic sequencing methods. The whole genome size of this strain was determined to be 1,813,536,357 bp, containing 3,902 coding genes. This strain contains 81 trnas, 79 RRnas and 194 repeat sequences. Analysis using the COG database indicated that 310 genes were involved in amino acid transport and metabolism, including 10 catabolic proteins related to COG. In the KEGG database, there are 201 genes involved in amino acid metabolic pathways, including 8 nitrogen metabolic pathway genes and 2 oxidation pathway genes. Subsequently, the antimicrobial peptide and secondary metabolite synthesis gene clusters of the LV149 genome were predicted, and the antimicrobial peptide gene for the synthesis of bacillocin 149 was screened out. In short, this study analyzed the antibacterial mechanism of LV149 through genomic sequencing technology and provided a theoretical basis for the subsequent development of probiotics. |
