| 项目编号 |
PRJCA047568 |
| 项目标题 |
Efficient Derivation of hiPSC-Derived Photoreceptor Precursor Cells and Their Neuroprotective Effects in Retinal Degeneration |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
Single cell sequencing
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Retinal degenerative diseases such as retinitis pigmentosa lack effective therapies capable of restoring vision. Photoreceptor precursor cell (PPC) transplantation has been identified as a potential solution to this issue. This study utilized PPCs efficiently derived from human induced pluripotent stem cells (hiPSCs). The use of AM580, a chemically stable retinoid analog, promoted the differentiation of hiPSCs into CRX-positive and LHX4-positive PPCs with 99.9 percent purity, ensuring the absence of off-target lineages. The retinal protective effects of these PPCs were demonstrated in an N-Methyl-N-nitrosourea-induced retinitis pigmentosa model, showing enhanced retinal intensity and spatial preservation. Donor PPCs expressed rod- and cone-specific markers and developed CtBP2-positive presynaptic specializations in close proximity to host bipolar cells. These results support the therapeutic potential of hiPSC-derived PPCs for retinal degeneration. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2025-10-28 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
|
|
Efficient Derivation of hiPSC-Derived Photoreceptor Precursor Cells and Their Neuroprotective Effects in Retinal Degeneration
|
iScience
|
10.1016/j.isci.2025.114196
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| iRegene Therapeutics Co., Ltd.
|
|
InternalRDFunding
|
|
|
|
| 提交者 |
Jingmin
Zhang (zhangjm@iregene.com)
|
| 提交单位 |
iRegene Therapeutics Co., Ltd |
| 提交日期 |
2025-10-09 |
