| 项目编号 |
PRJCA047963 |
| 项目标题 |
A small-molecule HSP90 inhibitor NVP-HSP990 alleviates rotavirus infection |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Rotavirus (RV) infection remains a leading cause of hospitalization and mortality among infants and young children. Despite global implementation of RV vaccines, tens of thousands of infants and young children still succumb to this disease each year due to effective treatment. In this study, we demonstrated that NVP-HSP990, a novel small-molecule heat shock protein 90 (HSP90) inhibitor, inhibited RV infection with a fascinating higher selectivity index (SI) compared to conventional HSP90 inhibitors like Geldanamycin (GA) and its derivative Tanespimycin (17-allylamino-17- demethoxygeldanamycin, 17-AAG). NVP-HSP990 effectively inhibited RV replication in vitro without blocking the initial establishment of infection. NVP-HSP990 restored host gene expression in most KEGG pathways disrupted by RV infection in Caco-2 cells except some inflammatory pathways (such as IL-17 and TNF pathways). NVP-HSP990 significantly inhibited RV-induced activation of the MAPK pathway and prevented the disruption of tight junctions in Caco-2 cells. More importantly, NVP-HSP990 effectively suppressed RV infection in BALB/c suckling mice and significantly alleviated RV-induced diarrhea. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2025-10-12 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Chongqing Municipal Basic and Frontier Research Project
|
|
cstc2015jcyjBX0086
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81570497
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32070884
|
|
|
|
| 提交者 |
Haiyang
He (ilovesea81@163.com)
|
| 提交单位 |
Army Medical University College of Basic Medical Sciences |
| 提交日期 |
2025-10-12 |
