| 项目编号 |
PRJCA050658 |
| 项目标题 |
Decreased Salivary ZG16B Expression Is Associated with Exocrine Dysfunction in Sjogren disease |
| 涉及领域 |
Medical |
| 数据类型 |
metabonomics
|
| 物种名称 |
|
| 描述信息 |
Saliva samples were thawed at room temperature, and 100 μL was aliquoted and mixed 1:1 with 0.06 mg/mL L-2-chlorophenylalanine (internal standard). Pre-chilled MeOH:ACN was introduced, followed by 1 min of vortexing and 10 min of ice-bath sonication; proteins were then precipitated at -20 °C for 30 min.After centrifugation at 13,000 rpm, the supernatants were taken to dryness; residues were reconstituted in methanol:water (1:4, v/v), sonicated for 3 min, incubated 2h at -20 °C, and centrifuged again. The resulting supernatants underwent 0.22-µm filtration and were frozen at -80 °C until assay. QC pools were assembled from equal parts of all specimens.LC was performed on a Waters ACQUITY UPLC I-Class Plus using an ACQUITY HSS T3 column. Mobile phase A (aqueous) was 0.1% formic acid in water; mobile phase B (organic) was 0.1% formic acid in acetonitrile. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2026-01-15 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 41310095
|
Decreased salivary ZG16B expression is associated with exocrine dysfunction in Sjögren disease
|
Clinical Rheumatology
|
10.1007/s10067-025-07841-x
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China grants
|
|
U21A20365
|
|
|
|
| 提交者 |
xiaoge
Sun (15055169238@163.com)
|
| 提交单位 |
Rheumatology And Immunology Department Of The First Affiliated Hospital Of Ustc |
| 提交日期 |
2025-11-09 |
