| Accession | PRJCA000889 |
|---|---|
| Title | monkey off-target |
| Umbrella project |
eGPS: evolutionary Genotype-Phenotype Systems biology Cranial Capacity & Structure Regulation of primate brain structure using Talen/Cas9 modified key genes |
| Data types | Whole genome sequencing |
| Organisms | Macaca mulatta |
| Description | The CRISPR-Cas9 system is a highly efficient genome editing tool widely used in model and non-model animals with promising potential in human gene therapy. Although with much improvement, the off-target effect of CRISPR-Cas9 remains a concern. Non-human primates (NHPs) share close genetic, immunological, physiological and neural similarity with humans, making them the ideal preclinical models for developing Cas9-based therapies. However, no robust off-target assessment has been conducted in NHPs. Here we performed trio sequencing of five Cas9-treated monkeys and their wild type parents to detect off-target effect of CRISPR-Cas9 system. |
| Sample scope | Multiisolate |
| Release date | 2018-10-01 |
| Submitter | Xin Luo (luoxin@mail.kiz.ac.cn) |
| Organization | Kunming Institute of Zoology, Chinese Academy of Sciences |
| Submission date | 2018-05-24 |
| Resource name (Count) | File Size (MB) |
| BioSample (10) | - |
| GSA (1) | - |
| CRA000954 | 753,594.35 |