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Accession PRJNA1065870
Title FoxO transcription factors actuate the formative pluripotency specific gene expression programme [RNA-Seq]
Relevance ModelOrganism
Data types Transcriptome or Gene expression
Sample scope Multiisolate
Organism Mus musculus [Taxonomy ID: 10090]
Description Naïve pluripotency is sustained by a self-reinforcing gene regulatory network (GRN) comprising core and naïve pluripotency-specific transcription factors (TFs). Upon exiting naïve pluripotency, ES cells transition through a formative post-implantation-like pluripotent state. However, the mechanisms underlying disengagement from the naïve GRN and initiation of the formative GRN remain unclear. Here, we demonstrate that phosphorylated AKT acts as a gatekeeper that prevents nuclear localization of FoxO TFs in naïve ESCs. PTEN-mediated reduction of AKT activity allows nuclear entry by FoxO TFs, enforcing a cell fate transition by binding and activating formative pluripotency-specific enhancers. Indeed, FoxO TFs are necessary and sufficient for transition from the naïve to the formative pluripotent state. Our work uncovers a pivotal role for FoxO TFs and AKT signalling in mechanisms underlying the exit from naïve pluripotency, a critical early embryonic cell fate transition. Overall design: Quantseq experiments from ES cells frown in 2i and in differentiation conditions in N2B27 medium; WT, Pten KO, Tsc2 KOs treated with DMSO and with a variety of inhibitorrs are used
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Organization Max Perutz Labs, University of Vienna
Data Source NCBI

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Experiment (26)  show -