Accession

PRJNA1065872

Title

FoxO transcription factors actuate the formative pluripotency specific gene expression programme [ChIP-Seq]

Relevance

ModelOrganism

Data types

Epigenomics

Sample scope

Multiisolate

Organism

Mus musculus [Taxonomy ID: 10090]

Description

Naïve pluripotency is sustained by a self-reinforcing gene regulatory network (GRN) comprising core and naïve pluripotency-specific transcription factors (TFs). Upon exiting naïve pluripotency, ES cells transition through a formative post-implantation-like pluripotent state. However, the mechanisms underlying disengagement from the naïve GRN and initiation of the formative GRN remain unclear. Here, we demonstrate that phosphorylated AKT acts as a gatekeeper that prevents nuclear localization of FoxO TFs in naïve ESCs. PTEN-mediated reduction of AKT activity allows nuclear entry by FoxO TFs, enforcing a cell fate transition by binding and activating formative pluripotency-specific enhancers. Indeed, FoxO TFs are necessary and sufficient for transition from the naïve to the formative pluripotent state. Our work uncovers a pivotal role for FoxO TFs and AKT signalling in mechanisms underlying the exit from naïve pluripotency, a critical early embryonic cell fate transition. Overall design: Chromatine-immunoprecipitation DNA sequencing (ChIP-seq) for FoxO1 and FoxO3 in WT and Pten KO mESC, in pluripotency supporting conditions (2i) and 24hours after 2i withdrawal (N24).

External link

Link description

Organization

Max Perutz Labs, University of Vienna

Data Source

NCBI

Project Data

Resource name	Description
Experiment (32) show	-
SRX23264952	GSM8021392: FoxO1, WT 2i, rep1, input; Mus musculus; ChIP-Seq
SRX23264953	GSM8021393: FoxO1, Pten KO 2i, rep1, input; Mus musculus; ChIP-Seq
SRX23264954	GSM8021394: FoxO1, WT 2i, rep2, input; Mus musculus; ChIP-Seq
SRX23264955	GSM8021395: FoxO1, Pten KO 2i, rep2, input; Mus musculus; ChIP-Seq
SRX23264956	GSM8021397: FoxO1, Pten KO N24, rep1, input; Mus musculus; ChIP-Seq
SRX23264957	GSM8021396: FoxO1, WT N24, rep1, input; Mus musculus; ChIP-Seq
SRX23264958	GSM8021398: FoxO1, WT N24, rep2, input; Mus musculus; ChIP-Seq
SRX23264959	GSM8021399: FoxO1, Pten KO N24, rep2, input; Mus musculus; ChIP-Seq
SRX23264960	GSM8021400: FoxO1, WT 2i, rep1, ChIP; Mus musculus; ChIP-Seq
SRX23264961	GSM8021401: FoxO1, Pten KO 2i, rep1, ChIP; Mus musculus; ChIP-Seq
SRX23264962	GSM8021402: FoxO1, WT 2i, rep2, ChIP; Mus musculus; ChIP-Seq
SRX23264963	GSM8021403: FoxO1, Pten KO 2i, rep2, ChIP; Mus musculus; ChIP-Seq
SRX23264964	GSM8021404: FoxO1, WT N24, rep1, ChIP; Mus musculus; ChIP-Seq
SRX23264965	GSM8021405: FoxO1, Pten KO N24, rep1, ChIP; Mus musculus; ChIP-Seq
SRX23264966	GSM8021406: FoxO1, WT N24, rep2, ChIP; Mus musculus; ChIP-Seq
SRX23264967	GSM8021407: FoxO1, Pten KO N24, rep2,ChIP; Mus musculus; ChIP-Seq
SRX23264968	GSM8021408: FoxO3, WT 2i, rep1, input; Mus musculus; ChIP-Seq
SRX23264969	GSM8021409: FoxO3, Pten KO 2i, rep1, input; Mus musculus; ChIP-Seq
SRX23264970	GSM8021410: FoxO3, WT N24, rep1, input; Mus musculus; ChIP-Seq
SRX23264971	GSM8021411: FoxO3, Pten KO N24, rep1, input; Mus musculus; ChIP-Seq
SRX23264972	GSM8021412: FoxO3, WT 2i, rep2, input; Mus musculus; ChIP-Seq
SRX23264973	GSM8021413: FoxO3, Pten KO 2i, rep2, input; Mus musculus; ChIP-Seq
SRX23264974	GSM8021414: FoxO3, WT N24, rep2, input; Mus musculus; ChIP-Seq
SRX23264975	GSM8021415: FoxO3, Pten KO N24, rep2, input; Mus musculus; ChIP-Seq
SRX23264976	GSM8021416: FoxO3, WT 2i, rep1, ChIP; Mus musculus; ChIP-Seq
SRX23264977	GSM8021417: FoxO3, Pten KO 2i, rep1, ChIP; Mus musculus; ChIP-Seq
SRX23264978	GSM8021418: FoxO3, WT N24, rep1, ChIP; Mus musculus; ChIP-Seq
SRX23264979	GSM8021419: FoxO3, Pten KO N24, rep1, ChIP; Mus musculus; ChIP-Seq
SRX23264980	GSM8021420: FoxO3, WT 2i, rep2, ChIP; Mus musculus; ChIP-Seq
SRX23264981	GSM8021421: FoxO3, Pten KO 2i, rep2, ChIP; Mus musculus; ChIP-Seq
SRX23264982	GSM8021422: FoxO3, WT N24, rep2, ChIP; Mus musculus; ChIP-Seq
SRX23264983	GSM8021423: FoxO3, Pten KO N24, rep2,ChIP; Mus musculus; ChIP-Seq