Accession PRJNA1069090
Title TLR-dependent transcriptional reprogramming of LXRα cistrome in bone marrow-derived macrophages [RNA-seq]
Relevance ModelOrganism
Data types Transcriptome or Gene expression
Sample scope Multiisolate
Organism Mus musculus [Taxonomy ID: 10090]
Description The Liver X receptors (LXRα and LXRβ) are members of the nuclear receptor superfamily of transcription factors, and play key roles in the coordination of both metabolic and immune responses in macrophages. Both LXRs are expressed in macrophages and they share a high degree of sequence homology but their individual roles in different models of macrophage cultures, particularly in response to inflammation, have not been characterized. Previous studies have shown that ligand pre-treated LXRs repress the induction of pro-inflammatory cytokines in macrophages. Here, we show evidence that the expression of LXRα (but not LXRβ) is transcriptionally regulated at late phases of an LPS-induced inflammatory response in bone marrow-derived macrophages (BMDMs). Regulation of LXRα expression is controlled at the transcriptional levels by inflammatory signaling networks. Transcriptional profiling studies and ChIP-seq data identified a number of innate immune pathways whose expression is substantially controlled by LXRα in BMDMs. In summary, our data shows that LXRα plays a previously unrecognized role in innate immunity by transcriptional regulation of a battery of inflammatory genes through TLR and Type-I IFN-dependent signaling in macrophages. Overall design: Expression profiling of peritoneal LPS-elicited macrophages (LXR-WT, LXR-aKO, LXR-bKO) treated with lipopolysaccharide 4 mg/Kg 24 hours.
PubMed ID Article title Journal name DOI Year
External link
Link description
Organization Lab B11, metabolism and cellular signaling, Instituto Investigaciones Biomedicas Alberto Sols CSIC
Data Source NCBI

Project Data

Resource name Description
Experiment (3) -
SRX23395273 GSM8036872: 02_LXR-aKO_LPS_24h; Mus musculus; RNA-Seq
SRX23395274 GSM8036873: 03_LXR-bKO_LPS_24h; Mus musculus; RNA-Seq
SRX23395272 GSM8036871: 01_LXR-WT_LPS_24h; Mus musculus; RNA-Seq