Accession

PRJNA1073179

Title

Sensory Flicker Stimulation of 5xFAD Mice Activates Immune Pathways in a Frequency and Time Dependent Manner

Relevance

ModelOrganism

Data types

Transcriptome or Gene expression

Sample scope

Multiisolate

Organism

Mus musculus [Taxonomy ID: 10090]

Description

We previously showed that exposing wild-type mice to 40Hz, but not 20Hz, visual flickering stimulation led to activation of MAPK and NFκB pathway signaling in the visual cortex within 15mins of stimulation and up-regulation of cytokines/chemokines that modulate microglia activity after 1hr. These observed frequency-dependent effects suggest that targeted frequencies, as well as durations of stimulation, hold the potential to fine-tune the effects of sensory stimulation on immune pathways, as well as possibly other pathways with the potential to correct the molecular changes associated with Alzheimer’s disease. To test this, we exposed 5xFAD mice and wild-type littermates to different frequencies of audio/visual flicker stimulation (ambient light, 10Hz, 20Hz, 40Hz) for durations of 0.5hr, 1hr, or 4hr, then used bulk RNAseq to profile transcriptional changes within the visual cortex and hippocampus. Within 5xFAD mice, we found that 1hr of stimulation yielded up-regulation immune-related pathways, but that these were no longer observed after 4hr of stimulation. In contrast, all three frequencies yielded consistent up-regulation of neurotransmission pathways at 1hr and 4hr. Importantly, a subset of genes sets were co-regulated between the visual cortex and hippocampus, suggesting that combined visual/audio flicker can target deep brain regions affected by Alzheimer’s disease. Collectively, our data indicate that specific durations and frequencies of audio/visual stimulation can be used to elicit particular changes in brain immunity and neuronal functions, supporting the importance of this non-invasive methodology to tune the brain for treatment of diverse neurological diseases Overall design: Wild-type and 5xFAD mice, visual cortex and hippocampus tissues. 26 experimental groups, 3 time points. 4 different frequencies ( Light (60Hz), 10Hz, 20Hz, and 40Hz) , N>=3

External link

Link description

Organization

Georgia Institute of Technology

Data Source

NCBI

Project Data

Resource name	Description
Experiment (176) show	-
SRX23527803	GSM8062405: WT 20Hz 1hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527804	GSM8062406: WT 20Hz 1hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527805	GSM8062407: WT 20Hz 1hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527806	GSM8062408: WT 20Hz 1hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527807	GSM8062409: WT 20Hz 1hr Rep7 Hip; Mus musculus; RNA-Seq
SRX23527808	GSM8062410: WT 20Hz 1hr Rep7 VC; Mus musculus; RNA-Seq
SRX23527809	GSM8062251: 5xFAD 10Hz 0.5hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527810	GSM8062252: 5xFAD 10Hz 0.5hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527811	GSM8062253: 5xFAD 10Hz 0.5hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527812	GSM8062254: 5xFAD 10Hz 0.5hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527813	GSM8062255: 5xFAD 10Hz 0.5hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527814	GSM8062256: 5xFAD 10Hz 0.5hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527815	GSM8062257: 5xFAD 10Hz 0.5hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527816	GSM8062258: 5xFAD 10Hz 0.5hr Rep4 VC; Mus musculus; RNA-Seq
SRX23527817	GSM8062275: 5xFAD 10Hz 1hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527818	GSM8062276: 5xFAD 10Hz 1hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527819	GSM8062277: 5xFAD 10Hz 4hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527820	GSM8062278: 5xFAD 10Hz 4hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527821	GSM8062279: 5xFAD 10Hz 4hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527822	GSM8062280: 5xFAD 10Hz 4hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527823	GSM8062281: 5xFAD 10Hz 4hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527824	GSM8062282: 5xFAD 10Hz 4hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527825	GSM8062307: 5xFAD 20Hz 1hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527826	GSM8062308: 5xFAD 20Hz 1hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527827	GSM8062309: 5xFAD 20Hz 1hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527828	GSM8062310: 5xFAD 20Hz 1hr Rep4 VC; Mus musculus; RNA-Seq
SRX23527829	GSM8062311: 5xFAD 20Hz 1hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527830	GSM8062312: 5xFAD 20Hz 1hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527831	GSM8062313: 5xFAD 20Hz 1hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527832	GSM8062314: 5xFAD 20Hz 1hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527833	GSM8062323: 5xFAD 20Hz 1hr Rep11 Hip; Mus musculus; RNA-Seq
SRX23527834	GSM8062324: 5xFAD 20Hz 1hr Rep11 VC; Mus musculus; RNA-Seq
SRX23527835	GSM8062325: 5xFAD 20Hz 1hr Rep12 Hip; Mus musculus; RNA-Seq
SRX23527836	GSM8062326: 5xFAD 20Hz 1hr Rep12 VC; Mus musculus; RNA-Seq
SRX23527837	GSM8062327: 5xFAD 20Hz 4hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527838	GSM8062328: 5xFAD 20Hz 4hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527839	GSM8062329: 5xFAD 20Hz 4hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527840	GSM8062330: 5xFAD 20Hz 4hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527841	GSM8062347: 5xFAD 40Hz 1hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527842	GSM8062348: 5xFAD 40Hz 1hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527843	GSM8062349: 5xFAD 40Hz 1hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527844	GSM8062350: 5xFAD 40Hz 1hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527845	GSM8062351: 5xFAD 40Hz 1hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527846	GSM8062352: 5xFAD 40Hz 1hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527847	GSM8062353: 5xFAD 40Hz 1hr Rep4 VC; Mus musculus; RNA-Seq
SRX23527848	GSM8062354: 5xFAD 40Hz 1hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527849	GSM8062379: 5xFAD 40Hz 4hr Rep4 VC; Mus musculus; RNA-Seq
SRX23527850	GSM8062380: 5xFAD 40Hz 4hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527851	GSM8062381: 5xFAD 40Hz 4hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527852	GSM8062382: 5xFAD 40Hz 4hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527853	GSM8062383: 5xFAD 40Hz 4hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527854	GSM8062384: 5xFAD 40Hz 4hr Rep7 Hip; Mus musculus; RNA-Seq
SRX23527855	GSM8062385: 5xFAD 40Hz 4hr Rep7 VC; Mus musculus; RNA-Seq
SRX23527856	GSM8062386: 5xFAD LightHz 1hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527857	GSM8062395: 5xFAD LightHz 1hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527858	GSM8062396: 5xFAD LightHz 1hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527859	GSM8062397: 5xFAD LightHz 1hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527860	GSM8062398: WT 20Hz 1hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527861	GSM8062399: WT 20Hz 1hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527862	GSM8062400: WT 20Hz 1hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527863	GSM8062401: WT 20Hz 1hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527864	GSM8062402: WT 20Hz 1hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527865	GSM8062419: WT 40Hz 1hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527866	GSM8062420: WT 40Hz 1hr Rep4 VC; Mus musculus; RNA-Seq
SRX23527867	GSM8062421: WT 40Hz 1hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527868	GSM8062422: WT 40Hz 1hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527869	GSM8062423: WT 40Hz 1hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527870	GSM8062424: WT 40Hz 1hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527871	GSM8062425: WT 40Hz 1hr Rep7 Hip; Mus musculus; RNA-Seq
SRX23527872	GSM8062426: WT 40Hz 1hr Rep7 VC; Mus musculus; RNA-Seq
SRX23527873	GSM8062259: 5xFAD 10Hz 0.5hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527874	GSM8062260: 5xFAD 10Hz 0.5hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527875	GSM8062261: 5xFAD 10Hz 0.5hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527876	GSM8062262: 5xFAD 10Hz 0.5hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527877	GSM8062263: 5xFAD 10Hz 0.5hr Rep7 Hip; Mus musculus; RNA-Seq
SRX23527878	GSM8062264: 5xFAD 10Hz 0.5hr Rep7 VC; Mus musculus; RNA-Seq
SRX23527879	GSM8062265: 5xFAD 10Hz 1hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527880	GSM8062266: 5xFAD 10Hz 1hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527881	GSM8062291: 5xFAD 20Hz 0.5hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527882	GSM8062292: 5xFAD 20Hz 0.5hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527883	GSM8062293: 5xFAD 20Hz 0.5hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527884	GSM8062294: 5xFAD 20Hz 0.5hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527885	GSM8062295: 5xFAD 20Hz 0.5hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527886	GSM8062296: 5xFAD 20Hz 0.5hr Rep4 VC; Mus musculus; RNA-Seq
SRX23527887	GSM8062297: 5xFAD 20Hz 0.5hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527888	GSM8062298: 5xFAD 20Hz 0.5hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527889	GSM8062315: 5xFAD 20Hz 1hr Rep7 Hip; Mus musculus; RNA-Seq
SRX23527890	GSM8062316: 5xFAD 20Hz 1hr Rep7 VC; Mus musculus; RNA-Seq
SRX23527891	GSM8062317: 5xFAD 20Hz 1hr Rep8 Hip; Mus musculus; RNA-Seq
SRX23527892	GSM8062318: 5xFAD 20Hz 1hr Rep8 VC; Mus musculus; RNA-Seq
SRX23527893	GSM8062319: 5xFAD 20Hz 1hr Rep9 Hip; Mus musculus; RNA-Seq
SRX23527894	GSM8062320: 5xFAD 20Hz 1hr Rep9 VC; Mus musculus; RNA-Seq
SRX23527895	GSM8062321: 5xFAD 20Hz 1hr Rep10 Hip; Mus musculus; RNA-Seq
SRX23527896	GSM8062322: 5xFAD 20Hz 1hr Rep10 VC; Mus musculus; RNA-Seq
SRX23527897	GSM8062339: 5xFAD 40Hz 0.5hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527898	GSM8062340: 5xFAD 40Hz 0.5hr Rep4 VC; Mus musculus; RNA-Seq
SRX23527899	GSM8062341: 5xFAD 40Hz 0.5hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527900	GSM8062342: 5xFAD 40Hz 0.5hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527901	GSM8062343: 5xFAD 40Hz 0.5hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527902	GSM8062344: 5xFAD 40Hz 0.5hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527903	GSM8062345: 5xFAD 40Hz 0.5hr Rep7 Hip; Mus musculus; RNA-Seq
SRX23527904	GSM8062346: 5xFAD 40Hz 1hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527905	GSM8062371: 5xFAD 40Hz 1hr Rep13 VC; Mus musculus; RNA-Seq
SRX23527906	GSM8062372: 5xFAD 40Hz 4hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527907	GSM8062373: 5xFAD 40Hz 4hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527908	GSM8062374: 5xFAD 40Hz 4hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527909	GSM8062375: 5xFAD 40Hz 4hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527910	GSM8062376: 5xFAD 40Hz 4hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527911	GSM8062377: 5xFAD 40Hz 4hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527912	GSM8062378: 5xFAD 40Hz 4hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527913	GSM8062411: WT 20Hz 1hr Rep8 Hip; Mus musculus; RNA-Seq
SRX23527914	GSM8062412: WT 20Hz 1hr Rep8 VC; Mus musculus; RNA-Seq
SRX23527915	GSM8062413: WT 40Hz 1hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527916	GSM8062414: WT 40Hz 1hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527917	GSM8062415: WT 40Hz 1hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527918	GSM8062416: WT 40Hz 1hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527919	GSM8062417: WT 40Hz 1hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527920	GSM8062418: WT 40Hz 1hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527921	GSM8062267: 5xFAD 10Hz 1hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527922	GSM8062268: 5xFAD 10Hz 1hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527923	GSM8062269: 5xFAD 10Hz 1hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527924	GSM8062270: 5xFAD 10Hz 1hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527925	GSM8062271: 5xFAD 10Hz 1hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527926	GSM8062272: 5xFAD 10Hz 1hr Rep4 VC; Mus musculus; RNA-Seq
SRX23527927	GSM8062273: 5xFAD 10Hz 1hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527928	GSM8062274: 5xFAD 10Hz 1hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527929	GSM8062299: 5xFAD 20Hz 0.5hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527930	GSM8062300: 5xFAD 20Hz 0.5hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527931	GSM8062301: 5xFAD 20Hz 0.5hr Rep7 Hip; Mus musculus; RNA-Seq
SRX23527932	GSM8062302: 5xFAD 20Hz 0.5hr Rep7 VC; Mus musculus; RNA-Seq
SRX23527933	GSM8062303: 5xFAD 20Hz 1hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527934	GSM8062304: 5xFAD 20Hz 1hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527935	GSM8062305: 5xFAD 20Hz 1hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527936	GSM8062306: 5xFAD 20Hz 1hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527937	GSM8062355: 5xFAD 40Hz 1hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527938	GSM8062356: 5xFAD 40Hz 1hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527939	GSM8062357: 5xFAD 40Hz 1hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527940	GSM8062358: 5xFAD 40Hz 1hr Rep7 Hip; Mus musculus; RNA-Seq
SRX23527941	GSM8062359: 5xFAD 40Hz 1hr Rep7 VC; Mus musculus; RNA-Seq
SRX23527942	GSM8062360: 5xFAD 40Hz 1hr Rep8 Hip; Mus musculus; RNA-Seq
SRX23527943	GSM8062361: 5xFAD 40Hz 1hr Rep8 VC; Mus musculus; RNA-Seq
SRX23527944	GSM8062362: 5xFAD 40Hz 1hr Rep9 Hip; Mus musculus; RNA-Seq
SRX23527945	GSM8062283: 5xFAD 10Hz 4hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527946	GSM8062284: 5xFAD 10Hz 4hr Rep4 VC; Mus musculus; RNA-Seq
SRX23527947	GSM8062285: 5xFAD 10Hz 4hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527948	GSM8062286: 5xFAD 10Hz 4hr Rep5 VC; Mus musculus; RNA-Seq
SRX23527949	GSM8062287: 5xFAD 10Hz 4hr Rep6 Hip; Mus musculus; RNA-Seq
SRX23527950	GSM8062288: 5xFAD 10Hz 4hr Rep6 VC; Mus musculus; RNA-Seq
SRX23527951	GSM8062289: 5xFAD 20Hz 0.5hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527952	GSM8062290: 5xFAD 20Hz 0.5hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527953	GSM8062331: 5xFAD 20Hz 4hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527954	GSM8062332: 5xFAD 20Hz 4hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527955	GSM8062333: 5xFAD 40Hz 0.5hr Rep1 Hip; Mus musculus; RNA-Seq
SRX23527956	GSM8062334: 5xFAD 40Hz 0.5hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527957	GSM8062335: 5xFAD 40Hz 0.5hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527958	GSM8062336: 5xFAD 40Hz 0.5hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527959	GSM8062337: 5xFAD 40Hz 0.5hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527960	GSM8062338: 5xFAD 40Hz 0.5hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527961	GSM8062363: 5xFAD 40Hz 1hr Rep9 VC; Mus musculus; RNA-Seq
SRX23527962	GSM8062364: 5xFAD 40Hz 1hr Rep10 Hip; Mus musculus; RNA-Seq
SRX23527963	GSM8062365: 5xFAD 40Hz 1hr Rep10 VC; Mus musculus; RNA-Seq
SRX23527964	GSM8062366: 5xFAD 40Hz 1hr Rep11 Hip; Mus musculus; RNA-Seq
SRX23527965	GSM8062367: 5xFAD 40Hz 1hr Rep11 VC; Mus musculus; RNA-Seq
SRX23527966	GSM8062368: 5xFAD 40Hz 1hr Rep12 Hip; Mus musculus; RNA-Seq
SRX23527967	GSM8062369: 5xFAD 40Hz 1hr Rep12 VC; Mus musculus; RNA-Seq
SRX23527968	GSM8062370: 5xFAD 40Hz 1hr Rep13 Hip; Mus musculus; RNA-Seq
SRX23527969	GSM8062387: 5xFAD LightHz 1hr Rep1 VC; Mus musculus; RNA-Seq
SRX23527970	GSM8062388: 5xFAD LightHz 1hr Rep2 Hip; Mus musculus; RNA-Seq
SRX23527971	GSM8062389: 5xFAD LightHz 1hr Rep2 VC; Mus musculus; RNA-Seq
SRX23527972	GSM8062390: 5xFAD LightHz 1hr Rep3 Hip; Mus musculus; RNA-Seq
SRX23527973	GSM8062391: 5xFAD LightHz 1hr Rep3 VC; Mus musculus; RNA-Seq
SRX23527974	GSM8062392: 5xFAD LightHz 1hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527975	GSM8062393: 5xFAD LightHz 1hr Rep4 VC; Mus musculus; RNA-Seq
SRX23527976	GSM8062394: 5xFAD LightHz 1hr Rep5 Hip; Mus musculus; RNA-Seq
SRX23527977	GSM8062403: WT 20Hz 1hr Rep4 Hip; Mus musculus; RNA-Seq
SRX23527978	GSM8062404: WT 20Hz 1hr Rep4 VC; Mus musculus; RNA-Seq