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项目编号 PRJNA1073278
项目标题 ADCY1-mediated cAMP signaling pathway mediates the neuropsychiatric effects of montelukast
涉及领域 Medical
数据类型 Transcriptome or Gene expression
样品范围 Multiisolate
生物名称 Homo sapiens [Taxonomy ID: 9606]
描述信息 Human forebrain organoids (day 100) derived from U2F iPS cell line were exposed with or without 20 ng/ml and 400 ng/ml montelukast (MTK) for 7 days. Organoids were then randomly selected for RNA sequencing (RNA-seq). By performing RNA-seq analysis, we showed that MTK exposure in hFOs downregulated expression of genes enriched with multiple neuronal functions including the glutamatergic synapse. These downregulated genes were also significantly overlapped with genes reported to be associated with schizophrenia, bipolar disorder, and autism. Overall design: We generated human forebrain organoids (hFOs) from U2F iPS cell lines. On day 100 of organoid culture, hFOs derived from U2F were exposed with or without for 20 ng/ml and 400 ng/ml MTK 7 days. Three organoids in each group were randomly pooled together as one mixed sample. Five mixed samples in each group were then used for RNA-seq (BGI DNBseq, paired-end 150 bp) to identify genes affected by MTK exposure. DEGs (adjusted P value < 0.05) that identified in hFOs derived from U2F were used for Gene Ontology and KEGG pathway analysis.
出版信息
PubMed ID 文章标题 杂志名称 Doi 发表年份
40471331
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发布日期 2025-06-16
提交单位 The First Affiliated Hospital of University of South China
数据来源 NCBI

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Experiment (15)  show -