| Description |
There is scarce information concerning the role of sporadic clones in the dissemination of antimicrobial resistance genes (ARG) within the nosocomial niche through the mechanisms of the Horizontal Genetic Transfer. In the present study, we used one of the first clinical mcr-1-harboring isolate from Latin America, E. coli M19736 ST 615. We confirmed its ability to receive crucial ARG by transformation and conjugation using as donors critical plasmids.Then, each transconjugant or transformant was evaluated for its ability to maintain the ARG on the first and 10th days in subcultures without antibiotic pressure. Finally, we isolated and characterized the EV by transmission electron microscopy, and dynamic light scattering that showed particles with a size 193 nm for E. coli M19736. Besides, by Liquid chromatography-MS-MS we studied the proteins that are inside of the EV from native E. coli M19736. We found 338 proteins in EV from native E. coli M19736 including the presence of the MCR-1 protein. These results evidenced the genetic plasticity of a sporadic clone of E. coli such as ST615 thatcould play a very important transitional link in the clinical dynamics and evolution to multidrug/extensively/pandrug phenotypes of superbugs within the nosocomial niche by acting as vector and reservoir of multiple ARG which later could be disseminated. |
