项目编号

PRJNA1152994

项目标题

Orchestration of pluripotent stem cell genome reactivation during mitotic exit [ChIP-seq]

涉及领域

ModelOrganism

数据类型

Epigenomics

样品范围

Multiisolate

生物名称

Mus musculus [Taxonomy ID: 10090]

描述信息

The maintenance of cell identity faces many challenges during mitosis, as most DNA-binding proteins are evicted from DNA and transcription is virtually abolished. How cells maintain their identity through cell division and faithfully re-initiate gene expression during mitotic exit is unclear. Here, we developed a novel reporter system enabling cell cycle synchronization-free separation of pluripotent stem cells in temporal bins of < 30 minutes during mitotic exit. This allowed us to quantify genome-wide reactivation of transcription, sequential changes in chromatin accessibility, and re-binding of the pluripotency transcription factors OCT4, SOX2, and NANOG (OSN). We found that transcriptional activity progressively ramped up after mitosis and that OSN rapidly reoccupied the genome during the anaphase-telophase transition. We also demonstrate transcription factor-specific, dynamic relocation patterns and a hierarchical reorganization of the OSN binding landscape governed by OCT4 and SOX2. Our study sheds light on the dynamic orchestration of transcriptional reactivation after mitosis. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) on OCT4, SOX2, and NANOG in different mESC lines under physiological conditions or in the precence or absence of doxycycline

出版信息

PubMed ID	文章标题	杂志名称	Doi	发表年份
40153434

项目相关网站链接

网站信息描述

发布日期

2025-11-19

提交单位

Suter, SV-IBI, EPFL

数据来源

NCBI

项目包含数据信息

资源名称	描述
Experiment (16) show	-
SRX25843498	GSM8485702: ChIP, Nanog, SOX2OFF, MDON, rep2; Mus musculus; ChIP-Seq
SRX25843499	GSM8485703: ChIP, Nanog, SOX2OFF, MDON, rep1; Mus musculus; ChIP-Seq
SRX25843500	GSM8485704: ChIP, Input, OCT4OFF, MDOFF, rep1; Mus musculus; ChIP-Seq
SRX25843501	GSM8485705: ChIP, Input, OCT4OFF, MDOFF, rep2; Mus musculus; ChIP-Seq
SRX25843502	GSM8485706: ChIP, Input, OCT4OFF, MDON, rep1; Mus musculus; ChIP-Seq
SRX25843503	GSM8485707: ChIP, Input, OCT4OFF, MDON, rep2; Mus musculus; ChIP-Seq
SRX25843504	GSM8485708: ChIP, Input, SOX2OFF, MDOFF, rep1; Mus musculus; ChIP-Seq
SRX25843505	GSM8485709: ChIP, Input, SOX2OFF, MDOFF, rep2; Mus musculus; ChIP-Seq
SRX25843506	GSM8485710: ChIP, Input, SOX2OFF, MDON, rep1; Mus musculus; ChIP-Seq
SRX25843507	GSM8485711: ChIP, Input, SOX2OFF, MDON, rep2; Mus musculus; ChIP-Seq
SRX25843508	GSM8485712: ChIP, Nanog, OCT4ON, MDOFF, rep1; Mus musculus; ChIP-Seq
SRX25843509	GSM8485713: ChIP, Nanog, OCT4ON, MDOFF, rep2; Mus musculus; ChIP-Seq
SRX25843510	GSM8485714: ChIP, Nanog, OCT4ON, MDON, rep1; Mus musculus; ChIP-Seq
SRX25843511	GSM8485715: ChIP, Nanog, OCT4ON, MDON, rep2; Mus musculus; ChIP-Seq
SRX25843512	GSM8485716: ChIP, Nanog, SOX2ON, MDON, rep1; Mus musculus; ChIP-Seq
SRX25843513	GSM8485717: ChIP, Nanog, SOX2ON, MDON, rep2; Mus musculus; ChIP-Seq