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项目编号 PRJNA1169620
项目标题 Transcriptional landscape of asymmetric gonadal development in female chicken embryos
涉及领域 Agricultural
数据类型 Transcriptome or Gene expression
样品范围 Multiisolate
生物名称 Gallus gallus [Taxonomy ID: 9031]
描述信息 Asymmetrical gonadal development is an intriguing phenomenon observed in the majority of female birds. In chickens, the left gonad of female embryos develops into a functional ovary, while the right gonad undergoes degeneration during embryogenesis. This sexually dimorphic trait is primarily induced by the spatial differential expression of the PITX2 gene. However, a comprehensive understanding of the transcriptional profile of the developing gonads during asymmetric development is still lacking. To elucidate the molecular mechanism of asymmetric gonadal development in chickens, we compared the transcriptomes between left and right gonads of female chickens using bulk- and single cell (sc) -RNA sequencing (RNA-seq) approaches. Our bulk RNA-seq analysis of the female chicken gonads at E5 (HH26), E6.5 (HH30), E8 (HH34), and E9.5 (HH36) revealed significant differential gene expression between the left and right female chicken gonads, particularly in signaling pathways, cell cycle, and metabolic processes. Moreover, scRNA-seq analysis revealed that coelomic epithelial, interstitial, and pre-granulosa cells of the left gonads share a highly proliferative status, contributing to the asymmetric gonadal cell proliferation, which may be regulated by the TGFβ signaling pathway. Our findings demonstrate that dynamic cell-type-specific transcriptional profiles during embryogenesis play a vital role in the asymmetric gonadal development of female chickens. Overall design: To investigate the female asymmetrical gonad development in avian species, we collected left and right embryonic gonads separately at four different time points during embryonic development and performed RNA-seq analysis
出版信息
PubMed ID 文章标题 杂志名称 Doi 发表年份
40014972
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发布日期 2025-09-26
提交单位 Penn State University
数据来源 NCBI

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