项目编号

PRJNA1303590

项目标题

Non-severe burn injury leads to sustained molecular changes in the brain in a murine model

涉及领域

ModelOrganism

数据类型

Transcriptome or Gene expression

样品范围

Multiisolate

生物名称

Mus musculus [Taxonomy ID: 10090]

描述信息

Background: Paediatric burn patients, including those with non-severe burns, have an increased risk of admission to hospital for mental health conditions for many years after the burn, even in children too young at the time of the burn to remember the incident. This study aimed to investigate the long-term physiological impact of non-severe burn injuries and non-burn trauma (NBT) on the brain in mice to understand whether there is a sustained impact of such injuries on the brain that may be linked to the increased mental health morbidity observed in patients. Methods: Mice were exposed to either a non-severe burn injury, an excision injury of the same size (equivalent non-burn trauma), or a sham procedure. Behavioural tests were conducted at multiple timepoints to measure anxiety and depression-like behaviour. Mice were euthanised three months after the injury, and plasma and brain tissue, including the hippocampus and prefrontal cortex, were isolated and examined using RNA sequencing, mass spectrometry and nuclear magnetic resonance (NMR) to identify transcriptomic and metabolomic changes. Results: A significant change in behaviour was observed with an increase in sucrose consumption three months after injury in the burn group compared to sham. Significant changes in the transcriptome were identified in some brain regions at 3 months after burn trauma compared to the sham group. Differentially expressed genes associated with inflammatory and immune functions were identified in the burn group compared to controls. Significant changes were also observed in the lipid profile and tryptophan catabolites in the brain after burn trauma compared to sham. Conclusion: Sustained changes in the transcriptome and metabolome were identified in a mouse model of non-severe burns, supporting a likely sustained pro-inflammatory environment in the brain after this type of injury. The potential link between these changes and the poor long-term mental health outcomes observed in paediatric burn patients requires further investigation. Overall design: This study investigated transcriptomic changes in the brain after burn injury by investigating three intervention groups: burn injury, excision injury, and sham, where RNA sequencing was done on three brain regions including the hippocampus, the frontal cortex and the piriform cortex. Two batches of hippocampus (n=14, n= 15) and frontal cortex (n=15, n=13) were analyzed along with one batch of piriform cortex (n=14). We performed gene and gene pathway analysis on these samples comparing the different intervention groups within the same region with batch corrections for the hippocampus and frontal cortex.

项目相关网站链接

网站信息描述

发布日期

2025-08-14

提交单位

Fiona Wood Foundation

数据来源

NCBI

项目包含数据信息

资源名称	描述
Experiment (71) show	-
SRX30046710	GSM9160228: Hippocampus, batch 1, excision, sample 1; Mus musculus; RNA-Seq
SRX30046711	GSM9160229: Hippocampus, batch 1, sham. sample 5; Mus musculus; RNA-Seq
SRX30046712	GSM9160230: Hippocampus, batch 1, burn, sample 1; Mus musculus; RNA-Seq
SRX30046713	GSM9160231: Hippocampus, batch 1, burn, sample 2; Mus musculus; RNA-Seq
SRX30046714	GSM9160232: Hippocampus, batch 1, burn, sample 3; Mus musculus; RNA-Seq
SRX30046715	GSM9160233: Hippocampus, batch 1, burn, sample 4; Mus musculus; RNA-Seq
SRX30046716	GSM9160234: Hippocampus, batch 1, burn, sample 5; Mus musculus; RNA-Seq
SRX30046717	GSM9160235: Hippocampus, batch 1, excision, sample 2; Mus musculus; RNA-Seq
SRX30046718	GSM9160236: Hippocampus, batch 1, excision, sample 3; Mus musculus; RNA-Seq
SRX30046719	GSM9160237: Hippocampus, batch 1, excision, sample 4; Mus musculus; RNA-Seq
SRX30046720	GSM9160238: Hippocampus, batch 1, excision, sample 5; Mus musculus; RNA-Seq
SRX30046721	GSM9160239: Hippocampus, batch 1, sham, sample 2; Mus musculus; RNA-Seq
SRX30046722	GSM9160240: Hippocampus, batch 1, sham, sample 3; Mus musculus; RNA-Seq
SRX30046723	GSM9160241: Hippocampus, batch 1, sham, sample 4; Mus musculus; RNA-Seq
SRX30046724	GSM9160242: Frontal cortex, batch 1, excision, sample 1; Mus musculus; RNA-Seq
SRX30046725	GSM9160243: Frontal cortex, batch 1, sham. sample 5; Mus musculus; RNA-Seq
SRX30046726	GSM9160244: Frontal cortex, batch 1, burn, sample 1; Mus musculus; RNA-Seq
SRX30046727	GSM9160245: Frontal cortex, batch 1, burn, sample 2; Mus musculus; RNA-Seq
SRX30046728	GSM9160246: Frontal cortex, batch 1, burn, sample 3; Mus musculus; RNA-Seq
SRX30046729	GSM9160247: Frontal cortex, batch 1, burn, sample 4; Mus musculus; RNA-Seq
SRX30046730	GSM9160248: Frontal cortex, batch 1, burn, sample 5; Mus musculus; RNA-Seq
SRX30046731	GSM9160249: Frontal cortex, batch 1, excision, sample 2; Mus musculus; RNA-Seq
SRX30046732	GSM9160250: Frontal cortex, batch 1, excision, sample 3; Mus musculus; RNA-Seq
SRX30046733	GSM9160251: Frontal cortex, batch 1, excision, sample 4; Mus musculus; RNA-Seq
SRX30046734	GSM9160252: Frontal cortex, batch 1, excision, sample 5; Mus musculus; RNA-Seq
SRX30046735	GSM9160253: Frontal cortex, batch 1, sham, sample 2; Mus musculus; RNA-Seq
SRX30046736	GSM9160254: Frontal cortex, batch 1, sham, sample 3; Mus musculus; RNA-Seq
SRX30046737	GSM9160255: Frontal cortex, batch 1, sham, sample 4; Mus musculus; RNA-Seq
SRX30046738	GSM9160256: Frontal cortex, batch 1, sham, sample 5; Mus musculus; RNA-Seq
SRX30046739	GSM9160257: Hippocampus, batch 2, burn, sample 1; Mus musculus; RNA-Seq
SRX30046740	GSM9160258: Hippocampus, batch 2, excision, sample 5; Mus musculus; RNA-Seq
SRX30046741	GSM9160259: Hippocampus, batch 2, sham, sample 1; Mus musculus; RNA-Seq
SRX30046742	GSM9160260: Hippocampus, batch 2, sham, sample 2; Mus musculus; RNA-Seq
SRX30046743	GSM9160261: Hippocampus, batch 2, sham, sample 3; Mus musculus; RNA-Seq
SRX30046744	GSM9160294: Frontal cortex, batch 3, excision, sample 4; Mus musculus; RNA-Seq
SRX30046745	GSM9160295: Frontal cortex, batch 3, excision, sample 5; Mus musculus; RNA-Seq
SRX30046746	GSM9160296: Frontal cortex, batch 3, sham, sample 2; Mus musculus; RNA-Seq
SRX30046747	GSM9160297: Frontal cortex, batch 3, sham, sample 3; Mus musculus; RNA-Seq
SRX30046748	GSM9160298: Frontal cortex, batch 3, sham, sample 4; Mus musculus; RNA-Seq
SRX30046749	GSM9160262: Hippocampus, batch 2, sham, sample 4; Mus musculus; RNA-Seq
SRX30046750	GSM9160263: Hippocampus, batch 2, sham, sample 5; Mus musculus; RNA-Seq
SRX30046751	GSM9160264: Hippocampus, batch 2, burn, sample 2; Mus musculus; RNA-Seq
SRX30046752	GSM9160265: Hippocampus, batch 2, burn, sample 3; Mus musculus; RNA-Seq
SRX30046753	GSM9160266: Hippocampus, batch 2, burn, sample 4; Mus musculus; RNA-Seq
SRX30046754	GSM9160267: Hippocampus, batch 2, burn, sample 5; Mus musculus; RNA-Seq
SRX30046755	GSM9160268: Hippocampus, batch 2, excision, sample 1; Mus musculus; RNA-Seq
SRX30046756	GSM9160269: Hippocampus, batch 2, excision, sample 2; Mus musculus; RNA-Seq
SRX30046757	GSM9160270: Hippocampus, batch 2, excision, sample 3; Mus musculus; RNA-Seq
SRX30046758	GSM9160271: Hippocampus, batch 2, excision, sample 4; Mus musculus; RNA-Seq
SRX30046759	GSM9160272: Frontal cortex, batch 2, burn, sample 1; Mus musculus; RNA-Seq
SRX30046760	GSM9160273: Frontal cortex, batch 2, excision, sample 5; Mus musculus; RNA-Seq
SRX30046761	GSM9160274: Frontal cortex, batch 2, sham, sample 1; Mus musculus; RNA-Seq
SRX30046762	GSM9160275: Frontal cortex,, batch 2, sham, sample 2; Mus musculus; RNA-Seq
SRX30046763	GSM9160276: Frontal cortex, batch 2, sham, sample 3; Mus musculus; RNA-Seq
SRX30046764	GSM9160277: Frontal cortex, batch 2, sham, sample 4; Mus musculus; RNA-Seq
SRX30046765	GSM9160278: Frontal cortex, batch 2, sham, sample 5; Mus musculus; RNA-Seq
SRX30046766	GSM9160279: Frontal cortex, batch 2, burn, sample 2; Mus musculus; RNA-Seq
SRX30046767	GSM9160280: Frontal cortex, batch 2, burn, sample 3; Mus musculus; RNA-Seq
SRX30046768	GSM9160281: Frontal cortex, batch 2, burn, sample 4; Mus musculus; RNA-Seq
SRX30046769	GSM9160282: Frontal cortex, batch 2, excision, sample 1; Mus musculus; RNA-Seq
SRX30046770	GSM9160283: Frontal cortex, batch 2, excision, sample 2; Mus musculus; RNA-Seq
SRX30046771	GSM9160284: Frontal cortex, batch 2, excision, sample 3; Mus musculus; RNA-Seq
SRX30046772	GSM9160285: Frontal cortex, batch 3, excision, sample 1; Mus musculus; RNA-Seq
SRX30046773	GSM9160286: Frontal cortex, batch 3, sham. sample 5; Mus musculus; RNA-Seq
SRX30046774	GSM9160287: Frontal cortex, batch 3, burn, sample 1; Mus musculus; RNA-Seq
SRX30046775	GSM9160288: Frontal cortex, batch 3, burn, sample 2; Mus musculus; RNA-Seq
SRX30046776	GSM9160289: Frontal cortex, batch 3, burn, sample 3; Mus musculus; RNA-Seq
SRX30046777	GSM9160290: Frontal cortex, batch 3, burn, sample 4; Mus musculus; RNA-Seq
SRX30046778	GSM9160291: Frontal cortex, batch 3, burn, sample 5; Mus musculus; RNA-Seq
SRX30046779	GSM9160292: Frontal cortex, batch 3, excision, sample 2; Mus musculus; RNA-Seq
SRX30046780	GSM9160293: Frontal cortex, batch 3, excision, sample 3; Mus musculus; RNA-Seq