To define molecular changes underlying this behavioral bias, we performed CUT&Tag on adult head/brain tissue to profile genome-wide occupancy of key chromatin features and/or associated factors, and mRNA-seq to quantify transcriptome responses. Integrating these epigenomic and transcriptomic datasets enables identification of core downstream targets and pathways associated with altered courtship-choice thresholds, providing a resource to study how epigenetic state interfaces with neuronal signaling and behavior.