| 项目编号 | PRJNA566173 |
|---|---|
| 项目标题 | Callithrix jacchus strain:cj1700 Genome sequencing and assembly |
| 数据类型 | Genome sequencing and assembly |
| 样品范围 | Monoisolate |
| 生物名称 | Callithrix jacchus [Taxonomy ID: 9483] |
| 描述信息 | The female white-tufted marmoset (Callithrix jacchus) DNA was derived from a cell line (cj1700) of a single skin biopsy. Sequences were generated on the Pacific Biosciences Sequel instrument (V2 chemistry) to approximately 70x genome coverage based on a genome size estimate of 3 Gb. All SMRT sequences were assembled with FALCON-Integrate v1.7.5 then error corrected using the Arrow error-correction module. Additional polishing of the assembly for residual indels was done by alignment of 40x coverage of Illumina data followed by application of the Pilon algorithm. Finally, residual indels were detected and corrected using a custom pipeline in the lab of Dr. Evan Eichler. All detected chimeric contigs were broken using automated and manual processes using a same DNA source BioNano map as guidance. Chromosomal level scaffolds/contig files were generated by alignment to Bionano mapping data, the human genome and marmoset/human maps (Mariano Rocchi), and to the prior Callithrix jacchus 3.2 reference. |
| 发布日期 | 2019-11-07 |
| 提交单位 | McDonnell Genome Institute at Washington University |
| 数据来源 | NCBI |
| 资源名称 | 描述 |
|---|---|
| Experiment (35) show | - |